高声压脉冲超声空化作用对兔肝细胞水通道蛋白4及钠钾ATP酶表达的影响  

Effects of acoustic cavitation on the expression of aquaporin 4 and sodium potassium ATP enzyme in rabbit hepatocytes

作　　者：皋月娟 王菲[2] 董小小 高文宏 GAO Yuejuan;WANG Fei;DONG Xiaoxiao;GAO Wenhong(Department of Ultrasound,the 5th Center of Chinese PLA General Hospital,Beijing 100039,China)

机构地区：[1]中国人民解放军总医院第五医学中心超声科,北京市100039 [2]昆明医科大学第一附属医院超声科 [3]中国人民解放军陆军军医大学第二附属医院超声科 [4]中国人民解放军中部战区总医院超声科

出　　处：《临床超声医学杂志》2021年第2期81-85,共5页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金项目(81301239);湖北省卫生健康科研基金项目(WJ2019H091)。

摘　　要：目的探讨高声压脉冲超声空化作用对兔肝细胞水通道蛋白4(AQP 4)及钠钾ATP酶(Na^+-K^+ATPase)表达的影响。方法将健康新西兰大白兔41只随机分为空白对照组(n=5)及不同辐照时间组:T1组(辐照时间1 min,n=12)、T5组(辐照时间5 min,n=12)及T10组(辐照时间10 min,n=12);T1、T5及T10组采用高峰值负压(P=2.0 MPa)脉冲超声联合经静脉脂质微泡注射行肝脏超声辐照,随后获取肝脏组织标本;空白对照组仅行开腹手术获取肝脏组织标本。免疫组织化学法观察各组AQP 4及Na^+-K^+ATPase蛋白表达分布情况;蛋白免疫印迹法检测各组AQP 4及Na^+-K^+ATPase蛋白表达量。结果超声辐照后T1、T5及T10组AQP 4及Na^+-K^+ATPase强阳性表达细胞均较空白对照组数量增多、分布范围广泛;AQP 4蛋白表达量均较空白对照组显著增高,差异均有统计学意义(均P<0.05),且T1、T10组与T5组AQP 4蛋白表达量比较差异均有统计学意义(均P<0.05),T1组与T10组比较差异无统计学意义;T5组和T10组Na^+-K^+ATPase蛋白表达量明显高于空白对照组及T1组,差异均有统计学意义(均P<0.05),T5组与T10组比较差异有统计学意义(P<0.05),T1组与空白对照组比较差异无统计学意义。结论高声压脉冲超声空化作用在引起正常兔肝脏组织水肿的同时可引起AQP 4及Na^+-K^+ATPase蛋白表达量的增加,超声辐照时间的长短可影响蛋白表达量。Objective To investigate the effects of acoustic cavitation on the expression of proteins-aquaporin 4(AQP 4)and sodium potassium ATP enzyme(Na^+-K^+ATPase)in normal rabbit hepatocytes.Methods Forty-one healthy New Zealand white rabbits were randomly divided into the control group(n=5)and three different irradiation time groups:T1 group(irradiation time was 1 min,n=12),T5 group(irradiation time was 5 min,n=12)and T10 group(irradiation time was 10 min,n=12).Animals in T1 group,T5 group and T10 group were treated with high peak negative pressure(P=2.0 MPa)ultrasound irradiation combined with microbubble.Liver tissue specimens were obtained after ultrasound irradiation.Liver tissue specimens were taken by laparotomy in the control group.Distribution of AQP 4 and Na^+-K^+ATPase in each group was observed by immunohistochemical method,and expression of both protein was detected by Western blot.Results AQP 4 and Na^+-K^+ATPase positive cells of treatment groups were more than those of control group.AQP 4 protein expression of T1 group,T5 group and T10 group were significantly higher than that of control group(all P<0.05).Comparison between groups showed that the expression of AQP 4 of T5 group was significantly higher than that of T1 group and T10 group(both P<0.05),while the difference between T1 group and T10 group was not statistically significant.The expression of Na^+-K^+ATPase of T5 group and T10 group were significantly higher than that of control group and T1 group(all P<0.05),while the difference between T1 group and control group was not statistically significant.Expression of Na^+-K^+ATPase in T5 group was significantly higher than that of T10 group(P<0.05).Conclusion Microbubble enhanced cavitation could increase expression of AQP 4 and Na^+-K^+ATPase in normal rabbit liver while induce liver edema,and ultrasonic irradiation time is closely related to protein expression.

关 键 词：超声空化 微泡 水通道蛋白 钠钾ATP酶 肝脏 兔 

分 类 号：R445.1[医药卫生—影像医学与核医学] R-33[医药卫生—诊断学]