妊娠母羊饲粮添加叶酸对不同出生类型新生羔羊脐带血管生成相关基因表达的影响  

Effects of Dietary Folic Acid Supplementation in Pregnant Ewes on Expression of Genes Related to Umbilical Cord Angiogenesis of Newborn Lambs of Different Litter Size

作　　者：彭思嘉 李鹤琼 罗海玲[1] 王波[1] 李贞 王月君 PENG Sijia;LI Heqiong;LUO Hailing;WANG Bo;LI Zhen;WANG Yuejun(State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学动物科技学院,动物营养学国家重点实验室,北京100193

出　　处：《动物营养学报》2021年第2期944-953,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发项目(2018YFD0500402);国家肉羊产业技术体系(CARS-38)。

摘　　要：试验旨在研究妊娠母羊饲粮添加叶酸对不同出生类型新生羔羊脐带血管生成相关基因表达的影响。选用体重相近、年龄一致的经产湖羊母羊120只,人工授精后随机分为3组,分别单栏饲喂补充0、16和32 mg/kg DM叶酸的全混合日粮。母羊分娩时,每组随机采集出生类型为双羔和三羔的胎盘与脐带样品各3个,共18个样品用于测定胎盘相关指标,胎儿脐带样品利用实时荧光定量PCR法检测血管生成相关基因——血管内皮生长因子A(VEGFA)、转换生长因子-β3(TGF-β3)、血管生成素-1(ANGPT-1)、血管内皮生长因子受体-1(FLT-1)和血管内皮生长因子受体-2(KDR)基因的相对表达水平。结果表明:妊娠母羊饲粮添加叶酸对胎盘重、子叶数、胎盘效率无显著影响(P>0.05),对新生羔羊脐带TGF-β3、ANGPT-1、FLT-1、KDR基因的相对表达水平无显著影响(P>0.05),但能显著提高VEGFA基因的相对表达水平(P<0.05)。而出生类型对胎盘重、子叶数、胎盘效率和新生羔羊脐带VEGFA、TGF-β3、FLT-1、KDR基因的相对表达水平无显著影响(P>0.05),但是三羔脐带ANGPT-1基因的相对表达水平显著高于双羔(P<0.05)。由此可见,妊娠母羊饲粮添加叶酸对胎盘发育没有显著影响,但是显著提高了新生羔羊脐带VEGFA基因的相对表达水平,有利于新生羔羊脐带血管生成。同时,新生羔羊三羔脐带ANGPT-1基因的相对表达水平显著高于双羔,有利于多羔脐带血管生成。This study aimed to investigate the effects of dietary folic acid supplementation in pregnant ewes on expression of genes related to umbilical cord angiogenesis of newborn lambs of different litter size.A total of 120 mutated Hu sheep ewes with similar body weight and consistent age were artificial inseminated and randomly divided into 3 groups,they were housed in individual pens and fed total mixed ration supplemented with 0,16 and 32 mg/kg DM folic acid,respectively.At the time of delivery,each 3 samples of placenta and fetal umbilical cord of twin lambs and triplet lambs were collected randomly from each group,a total of 18 samples were used to measure placental related traits.The real-time quantitative PCR was used to detect the relative expression levels of the angiogenesis-related genes,including vascular endothelial growth factor A(VEGFA),transforming growth factor-β3(TGF-β3),angiopoietin-1(ANGPT-1),fms related tyrosine kinase 1(FLT-1)and kinase insert domain receptor(KDR).The results showed that the dietary folic acid supplementation in pregnant ewes had no significant effects on placental weight,cotyledon number,placental efficiency(P>0.05),and had no significant effects on the relative expression levels of TGF-β3,ANGPT-1,FLT-1 and KDR genes in the umbilical cord of newborn lambs(P>0.05),but significantly increased the relative expression level of VEGFA gene(P<0.05).Besides,litter size had no significant effects on placental weight,cotyledon number,placental efficiency and the relative expression levels of VEGFA,TGF-β3,FLT-1 and KDR genes in the umbilical cord of newborn lambs(P>0.05),while the relative expression level of ANGPT-1 gene of triplet lambs was significantly higher than that of twin lambs(P<0.05).In conclusion,the dietary folic acid supplementation in pregnant ewes have no significant effects on the development of placenta,but it can significantly increase the relative expression level of VEGFA gene in the umbilical cord of newborn lambs,which is beneficial to umbilical cord angiogenesis

关 键 词：母羊 叶酸 脐带 血管生成 基因表达 

分 类 号：S826[农业科学—畜牧学]