包膜丁酸钠对脂多糖刺激下断奶羔羊血清炎性细胞因子、肠道通透性及肠道组织形态的影响  

Effects of Coated Sodium Butyrate on Serum Inflammatory Cytokines,Intestinal Permeability and Intestinal Morphology of Weaned Lambs Challenged with Lipopolysaccharide

作　　者：王可鑫 左丽君 陈想 姜宁[1] 潘春媛[1] 李凌岩[1] 张爱忠[1] WANG Kexin;ZUO Lijun;CHEN Xiang;JIANG Ning;PAN Chunyuan;LI Lingyan;ZHANG Aizhong(Key Laboratory of Feed Resource Efficient Utilization and Nutrition Manipulation in Cold Region of Heilongjiang Province,College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江省寒区饲料资源高效利用与营养调控重点实验室,大庆163319

出　　处：《动物营养学报》2021年第2期954-964,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：黑龙江省自然科学基金项目(LH2019C053)。

摘　　要：本试验旨在研究包膜丁酸钠(CSB)对脂多糖(LPS)刺激下断奶羔羊血清炎性细胞因子、肠道通透性及肠道组织形态的影响。试验选取(42±1)日龄、平均体重为(11.79±0.54)kg的断奶羔羊24只,按体重相近原则随机分为4组:空白组(CON组)、LPS组、CSB2L组、CSB3L组,其中CON和LPS组饲喂基础饲粮,CSB2L和CSB3L组在基础饲粮中分别添加2和3 g/kg CSB,每组6个重复,每个重复1只羊。在试验第28天每组选取3只羔羊进行屠宰,LPS、CSB2L和CSB3L组在屠宰前3 h腹腔注射100μg/kg BW LPS,CON组腹腔注射等量无菌生理盐水。结果表明:1)与CON组相比,饲粮中添加CSB可显著提高断奶羔羊的平均日采食量(ADFI)(P<0.05),CSB3L组的平均日增重(ADG)显著高于CON和LPS组(P<0.05)。2)与CON组相比,LPS组断奶羔羊血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和白细胞介素-8(IL-8)含量显著升高(P<0.05)。CSB2L和CSB3L组血清TNF-α和IL-1β含量均低于LPS组(P<0.05),血清TNF-α含量显著高于CON组(P<0.05)。CSB3L组血清IL-8含量显著低于LPS组(P<0.05)。CSB2L和CSB3L组血清白细胞介素-10(IL-10)含量显著高于LPS组(P<0.05)。3)断奶羔羊血清中D-乳酸(DLA)含量和二胺氧化酶(DAO)活性在LPS刺激下显著升高(P<0.05)。CSB2L和CSB3L组可显著降低LPS注射后的血清DLA含量和DAO活性(P<0.05)。4)CON组肠黏膜形态结构完整且绒毛排列整齐。注射LPS后,空肠和回肠绒毛排列紊乱,高矮不一,绒毛肿胀脱落。CSB2L和CSB3L组空肠和回肠绒毛形态较完整且排列整齐,肿胀程度降低,上皮轻度脱落。LPS组盲肠部分肠绒毛上皮脱落,腺体排列紊乱,结肠绒毛略微脱落。CSB2L组盲肠和结肠有轻微水肿,CSB3L组盲肠和结肠绒毛上皮未见明显脱落和水肿。LPS刺激显著降低了空肠和回肠绒毛高度和绒毛高度/隐窝深度(P<0.05)。CSB2L和CSB3L组空肠和回肠绒毛高度降低和绒毛高度/隐窝深度降低均得到缓解。CSB2L和CSB3L组十�This experiment was conducted to investigate the effects of coated sodium butyrate(CSB)on serum inflammatory cytokines,intestinal permeability and intestinal morphology in weaned lambs challenged with lipopolysaccharide(LPS).Twenty-four weaned lambs with an average body weight(11.79±0.54)kg were randomly allocated to 4 groups with 6 replicates per group and 1 lamb per replicate according to similar body weight:control group(CON),LPS group,CSB2L group and CSB3L group.CON and LPS groups were fed a basal diet,CSB2L and CSB3L groups were supplemented with 2 and 3 g/kg CSB,respectively.Three lambs in each group were slaughtered on the 28th day.LPS,CSB2L and CSB3L groups were intraperitoneally injected with 100μg/kg BW LPS,and CON group was intraperitoneally injected with the same amount of sterile normal saline 3 h before slaughter on the 28th day.The results showed as follows:1)the addition of CSB to the diet could significantly increase average daily feed intake(ADFI)of weaned lambs(P<0.05).The average daily gain(ADG)in CSB3L group was significantly higher than that in CON group or LPS group(P<0.05).2)Compared with CON group,the contents of tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-8 in serum of weaned lambs were significantly higher in LPS group(P<0.05).The contents of serum TNF-αand IL-1βin CSB2L and CSB3L groups were significantly lower than those in LPS group(P<0.05),and the content of serum TNF-αwas significantly higher than that in CON group(P<0.05).The content of serum IL-8 in CSB3L group was significantly lower than that in LPS group(P<0.05).The content of IL-10 in CSB2L and CSB3L groups was significantly higher than that in LPS group(P<0.05).3)LPS challenge significantly increased the D-lactate(DLA)content and diamine oxidase(DAO)activity in serum of weaned lambs(P<0.05).CSB2L and CSB3L groups can significantly decreased the DLA content and DAO activity after LPS challenge(P<0.05).4)The morphology and structure of intestinal mucosa were intact and the villi were arranged neatly in CON

关 键 词：包膜丁酸钠 断奶羔羊 脂多糖 肠道健康 

分 类 号：S826[农业科学—畜牧学]