白藜芦醇对氧化应激仔猪回肠黏膜形态、抗氧化能力、紧密连接蛋白及炎性因子mRNA表达的影响  

Effects of Resveratrol on Ileal Mucosal Morphology,Antioxidant Capacity,Tight Junction Protein and Inflammatory Factor mRNA Expression in Oxidative Stress Piglets

作　　者：符清瑶 施力光[2] 周汉林[2] 曹婷[2] 马中华 姜宏正 荀文娟 FU Qingyao;SHI Liguang;ZHOU Hanlin;CAO Ting;MA Zhonghua;JIANG Hongzheng;XUN Wenjuan(College of Animal Science and Technology,Hainan University,Haikou 570228,China;Institute of Tropical Crop Variety Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737,China)

机构地区：[1]海南大学动物科技学院,海口570228 [2]中国热带农业科学院热带作物品种资源研究所,儋州571737

出　　处：《动物营养学报》2021年第2期1163-1172,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：中国热带农业科学院基本科研业务费专项资金(1630032019023,1630032017035);国家农业农村部农产品质量安全专项(2130109);海南省自然科学基金青年基金项目(319QN307)。

摘　　要：本试验旨在研究白藜芦醇(Res)对氧化应激仔猪肠道黏膜结构、抗氧化能力、紧密连接蛋白及炎性因子mRNA表达的影响。选择健康状况良好、胎次相近的28日龄三元杂交(杜×长×大)断奶仔猪30头,随机分为5组,对照组饲喂基础饲粮,试验组在基础饲粮中分别添加0、10、30、90 mg/kg Res,每组6个重复,每个重复1头仔猪。于试验第15天清晨给试验组[敌草快(Diquat)组、Diquat+10 mg/kg Res组、Diquat+30 mg/kg Res组、Diquat+90 mg/kg Res组]仔猪按照10 mg/kg BW的剂量腹腔注射Diquat建立氧化应激模型,对照组只注射相同剂量灭菌生理盐水为参照。试验期21 d。结果表明:与对照组相比,Diquat组绒毛高度和绒毛高度/隐窝深度(V/C)显著降低(P<0.05),隐窝深度显著升高(P<0.05);肠黏膜丙二醛(MDA)含量显著升高(P<0.05),谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性显著降低(P<0.05);肠黏膜白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量显著升高(P<0.05),而肠黏膜白细胞介素-10(IL-10)含量显著降低(P<0.05);肠黏膜闭锁小带蛋白-1(ZO-1)、闭锁蛋白(Occludin)和跨膜蛋白(Claudin)的mRNA相对表达量均显著降低(P<0.05)。与Diquat组相比,Diquat+30 mg/kg Res组和Diquat+90 mg/kg Res组回肠绒毛高度和V/C显著提高(P<0.05);肠黏膜MDA含量显著下降(P<0.05),GSH-Px和SOD活性显著提高(P<0.05),其中以Diquat+90 mg/kg Res组效果最为显著;肠黏膜ZO-1、Occludin和Claudin的mRNA相对表达量均显著提高(P<0.05),且不同Res剂量组间无显著差异(P>0.05);肠黏膜IL-1β、TNF-α和IL-6含量显著降低(P<0.05),同时IL-10含量显著提高(P<0.05)。综上所述,Res可以提高氧化应激仔猪回肠抗氧化酶活性,改善回肠黏膜形态结构,同时抑制肠道炎症的发生,维持肠道健康。The purpose of this experiment was to study resveratrol(Res)on the intestinal mucosal structure,antioxidant capacity,tight junction protein and inflammatory factor mRNA expression in oxidative stress piglets.Thirty 28-day-old three-way cross(Duroc×Landrace×Yorkshire)weaned piglets with good health and similar parity were selected and randomly divided into 5 groups,with 6 replicates in each group and 1 piglet in each replicate.The control group was fed a basal diet,and the experimental groups were intraperitoneally injected with Diquat+Res of 0,10,30 and 90 mg/kg were added to the basal diet,respectively.In the early morning of the 15th day of the experiment,the piglets in the experimental groups(Diquat group,Diquat+10 mg/kg Res group,Diquat+30 mg/kg Res group and Diquat+90 mg/kg Res group)were intraperitoneally injected with Diquat at a dose of 10 mg/kg BW to establish an oxidative stress model,and the control group only injected the same dose of sterilized saline as a reference.The trial period was 21 days.The results showed that:compared with the control group,the villus height and the ratio of villus height/crypt depth(V/C)of the Diquat group were significantly reduced(P<0.05),and the crypt depth was significantly increased(P<0.05);the content of malondialdehyde(MDA)increased significantly(P<0.05),the activity of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)were significantly reduced in intestinal mucosa(P<0.05),intestinal mucosal interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)contents were significantly increased(P<0.05),while the intestinal mucosal interleukin-10(IL-10)content was significantly decreased(P<0.05);the relative expression levels of zonules atresia protein-1(ZO-1),Occludin and transmembrane protein(Claudin)in the intestinal mucosa were significantly decreased(P<0.05).Compared with the Diquat group,the ileal villi height and V/C in the Diquat+30 mg/kg Res group and Diquat+90 mg/kg Res group were significantly increased(P<0.05);the MDA content of the

关 键 词：白藜芦醇 仔猪 回肠黏膜 氧化应激 肠道健康 

分 类 号：S828[农业科学—畜牧学]