超快速液相色谱-三重四级杆/线性离子阱质谱法同时测定鸡血藤不同产地及商品药材中多元活性成分  

Simultaneous Determination of Multiple Bioactive Constituents in Spatholobi Caulis from Different Habitats and Commercial Herbs by UFLC-QTRAP-MS/MS

作　　者：梅余琪 魏丽芳 邹立思[1] 刘训红[1] 陈佳丽 谈梦霞 王程成 蔡芷辰[1] 张芙蓉 MEI Yu-qi;WEI Li-fang;ZOU Li-si;LIU Xun-hong;CHEN Jia-li;TAN Meng-xia;WANG Cheng-cheng;CAI Zhi-chen;ZHANG Fu-rong(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,南京210023

出　　处：《中国药学杂志》2020年第24期2012-2020,共9页Chinese Pharmaceutical Journal

基　　金：江苏高校优势学科建设工程项目资助(YSXK-2014);江苏高校品牌专业建设工程项目资助(PPZY2015A070)。

摘　　要：目的建立超快速液相色谱-三重四级杆/线性离子阱质谱法(UFLC-QTRAP-MS/MS)同时测定鸡血藤不同产地及商品药材中黄烷醇、异黄酮、黄酮醇、二氢黄酮、二氢黄酮醇、查耳酮、紫檀烷、花色素和酚酸类共22种活性成分含量的方法;根据22种目标成分的含量,用主成分分析对不同产地的鸡血藤药材进行综合评价。方法采用XBridge C18柱(4.6 mm×100 mm, 3.5μm),以0.3%甲酸水-甲醇为流动相,梯度洗脱,流速0.8 mL·min^-1,柱温30℃,多反应监测离子扫描模式(MRM)测定;根据22种目标成分的含量,用主成分分析(PCA)对所检测的31个批次鸡血藤药材进行综合评价。结果 22种目标成分的浓度与峰面积的线性关系良好(r>0.999 0);平均加样回收率为97.95%~103.1%,RSD均小于5%。结论所建立的方法准确、可靠,可用于鸡血藤中多元功效物质或指标成分的同时测定,为鸡血藤药材内在质量的综合评价和全面控制提供新的方法参考。OBJECTIVE To develop a method for simultaneous determination of flavanols, isoflavones, flavonols, dihydroflavones, flavanonols, chalcones, pterocarpan, anthocyanidins and phenolic acids in Spatholobi Caulis from different habitats and commercial herbs by UFLC-QTRAP-MS/MS. Principal component analysis(PCA) was applied to comprehensively analyze and evaluate the results of multiple active constituents. METHODS The chromatographic separation was performed on an XBridge C18 column(4.6 mm×100 mm, 3.5 μm) at 30 ℃ with gradient elution by 0.3% formic acid aqueous solution-methanol, and the flow rate was 0.8 mL·min^-1, using multiple-reaction monitoring(MRM) mode. Principal component analysis(PCA) was performed to evaluate 31 samples according to the contents of 22 constituents. RESULTS The 22 target components showed good linearity(r>0.999 0) in the range of the tested concentrations. The average recovery rates of the 22 components were from 97.46% to 103.6% with relative standard deviations less than 5%. CONCLUSION The developed method is accurate and reliable, and could be used for simultaneous determination of multi-functional substances or index components in Spatholobi Caulis, which could provide a reference for the comprehensive evaluation and quality control of Spatholobi Caulis.

关 键 词：超快速液相色谱-三重四级杆/线性离子阱质谱法 鸡血藤 多元活性成分 主成分分析 黄酮 酚酸 

分 类 号：R284[医药卫生—中药学]