云南省祥云县蚊虫携带病毒的调查研究  

Investigation of mosquito-borne viruses in Xiangyun county, Yunnan province

作　　者：潘虹[1] 付冬梅 冯云[1] 杨卫红[1] 李江春 Pan Hong;Fu Dongmei;Feng Yun;Yang Weihong;Li Jiangchun(Department of Viral Disease and Rickettsiosis Prevention and Control,Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention,Yunnan Institute of Endemic Disease Control and Prevention,Dali 671000,China;School of Public Health,Kunming Medical University,Kunming 650500,China)

机构地区：[1]云南省地方病防治所病毒立克次体病防治科、云南省自然疫源性疾病防控技术重点实验室,大理671000 [2]昆明医科大学公共卫生学院,650500

出　　处：《国际病毒学杂志》2020年第6期484-487,共4页International Journal of Virology

基　　金：国家自然科学基金项目(No.31560049);云南省高层次卫生计生技术人才-云南省医学学科带头人(D-2017055);云南省中青年学术和技术带头人后备人才(2019HB052)。

摘　　要：目的调查云南省大理白族自治州祥云县蚊虫中蚊媒病毒携带情况。方法2017年8月在祥云县水涨地、清华洞捕蚊,对蚊虫分类鉴定、计数,接种至BHK-21细胞分离病毒,阳性分离物用RT-PCR扩增序列,鉴定分析病毒种类和分子特征。结果共捕获蚊虫20276只,其中86.87%为三带喙库蚊,7.89%为按蚊,1.29%为致倦库蚊,3.95%为其他未分类蚊种。病毒分离后获得阳性分离物17株,均为乙脑病毒I型,其中16株来自三带喙库蚊,1株来自按蚊。结论三带喙库蚊为祥云县的主要优势蚊种和带毒蚊种;首次在祥云县分离到乙脑病毒,且均为基因I型。Objective To investigate the status of mosquito-borne viruses in mosquitoes in Xiangyun county of Dali Bai Autonomous Prefecture,Yunnan province.Methods Mosquitoes were caught in Shuizhangdi village and Qinghua cave in Xiangyun county in August 2017.The mosquito specimens were classification,identified and counted.The specimens were innoculated in BHK-21 cells to isolate the virues.Postive cultures were used to amplify gene sequences with RT-PCR.The species and molecular characteristics of the viruses were then analyzed.Results A total of 20276 mosquitoes were caught.Culex tritaeniorhynchus accounted for 86.87%,Anopheles accounted for 7.89%,Cx.pipiens quinquefasciatus accounted for 1.29%and others unidentified mosquitos accounted for 3.95%.Seventeen positive cultures were obtained and RT-PCR results indicated that all strains were Japanese encephalitis virus genotype I,including one stain from Anophele and 16 from Culex tritaeniorhynchus.Conclusions Culex tritaeniorhynchus is the predominant and virus-carrying mosquitoes species in Xiangyun county.This is the first time to isolate Japanese encephalitis virus in mosquitoes of Xiangyun county,and all strains were genotype I.

关 键 词：蚊虫 乙脑病毒 分子特征 

分 类 号：R181.3[医药卫生—流行病学]