Comparison of developmental toxicity of different surface modified CdSe/ZnS QDs in zebrafish embryos  

作　　者：Naying Zheng Jinhui Yan Wang Qian Chao Song Zhenghong Zuo Chengyong He 

机构地区：[1]State Key Laboratory of Cellular Stress Biology,School of Life Sciences,Xiamen University,Xiamen 361005,China [2]Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wixi 214081,China

出　　处：《Journal of Environmental Sciences》2021年第2期240-249,共10页环境科学学报（英文版）

基　　金：the National Natural Science Foundation of China(No.31971234);the Fundamental Research Funds for the Central Universities(No.20720180045);the Open Research Fund of State Key Laboratory of Cellular Stress Biology,Xiamen University(No.SKLCSB2019KF001)。

摘　　要：Quantum dots(QDs)are new types of nanomaterials.Few studies have focused on the effect of different surface modified QDs on embryonic development.Herein,we compared the in vivo toxicity of Cd Se/Zn S QDs with carboxyl(-COOH)and amino(-NH 2)modification using zebrafish embryos.After exposure,the two Cd Se/Zn S QDs decreased the survival rate,hatching rate,and embryo movement of zebrafish.Moreover,we found QDs attached to the embryo membrane before hatching and the eyes,yolk and heart after hatching.The attached amount of carboxyl QDs was more.Consistently,the Cd content in embryos and larvae was higher in carboxyl QD-treatment.We further observed that the two QDs caused zebrafish pericardial edema and cardiac dysfunction.In line with it,both carboxyl and amino QDs upregulated the transcription levels of cardiac development-related genes,and the levels were higher in carboxyl QD-treated groups.Furthermore,the chelator of Cd^2+diethylene triamine pentacetate acid could partially rescued the developmental toxicity caused by the two types of QDs suggesting that both the nature of QDs and the release of Cd^2+contribute to the developmental toxicity.In conclusion,the two Cd Se/ZnS QDs have developmental toxicity and affect the cardiac development,and the carboxyl QDs is more toxic possibly due to the higher affinity and more release to embryos and larvae.Our study provides new knowledge that the surface functional modification of QDs is critical on the development on aquatic species,which is beneficial to develop and applicate QDs more safely and environmentfriendly.

关 键 词：Quantum dots Surface modification FISH Cardiac development Developmental toxicity 

分 类 号：X503.225[环境科学与工程—环境工程] TB383.1[一般工业技术—材料科学与工程]