减重平板训练对脊髓损伤大鼠神经病理性疼痛及脊髓后角谷氨酸脱羧酶-65/67表达的影响  

Effects of Body Weight-supported Treadmill Training on Neuropathic Pain and Expression of Glutamate Decarboxylase(GAD)-65/67 in Spinal Dorsal Horn of Rats with Spinal Cord Injury

作　　者：李向哲 丁洁 王庆华 董传明[4] 王彤 吴勤峰 LI Xiang-zhe;DING Jie;WANG Qing-hua;DONG Chuan-ming;WANG Tong;WU Qin-feng(the Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University,Suzhou,Jiangsu 215153,China;Sir Run Run Shaw Hospital of Zhejiang University School of Medicine,Hangzhou,Zhejiang 310016,China;Experimental Animal Center of Nantong University,Nantong,Jiangsu 226001,China;Medical College of Nantong University,Nantong,Jiangsu 226001,China;Rehabilitation Medical Center,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China)

机构地区：[1]南京医科大学附属苏州科技城医院,江苏苏州市215153 [2]浙江大学医学院附属邵逸夫医院,浙江杭州市310016 [3]南通大学实验动物中心,江苏南通市226001 [4]南通大学医学院,江苏南通市226001 [5]南京医科大学第一附属医院康复医学中心,江苏南京市210029

出　　处：《中国康复理论与实践》2021年第2期131-136,共6页Chinese Journal of Rehabilitation Theory and Practice

基　　金：苏州市"科教兴卫"青年科技项目(No.KJXW2018082);苏州市科技局民生科技项目(No.SYS201785,No.SYS2019016);国家自然科学基金面上项目(No.81672258)。

摘　　要：目的探讨减重平板训练对脊髓损伤大鼠神经病理性疼痛及脊髓后角内谷氨酸脱羧酶-65/67(GAD-65/67)表达的影响。方法将24只Sprague-Dawley大鼠随机分为假手术组(Sham组)、脊髓损伤-不运动组(SCI-Sed组)和脊髓损伤-运动组(SCI-Ex组),每组8只。采用Allen法制作T10不完全性脊髓损伤模型。在脊髓损伤后7 d对SCI-Ex组进行减重平板训练。于脊髓损伤前,脊髓损伤后7 d、14 d、21 d、28 d和35 d,分别采用von Frey细丝和热刺激痛觉测试仪进行机械性痛阈和热痛阈评估。痛阈评估结束后,采用Western blotting和免疫组化染色检测所有大鼠腰髓GAD-65和GAD-67。结果脊髓损伤后21 d、28 d和35 d,SCI-Ex组机械性痛阈和热痛阈均明显高于SCI-Sed组(P<0.01)。与Sham组相比,SCI-Sed组和SCI-Ex组GAD-65和GAD-67表达减少(P<0.05);与SCI-Sed组相比,SCI-Ex组GAD-65和GAD-67表达量升高(P<0.05)。结论减重平板训练可增加不完全性脊髓损伤大鼠损伤远端脊髓后角GAD-65/67的合成,并改善大鼠后肢痛阈。Objective To investigate the effect of body weight-supported treadmill training on neuropathic pain and expression of glutamate decarboxylase-65/67(GAD-65/67)in spinal dorsal horn of rats with spinal cord injury(SCI).Methods A total of 24 Sprague-Dawley rats were randomly divided into sham group,SCI-sedentary(SCI-Sed)group and SCI-Exercise(SCI-Ex)group,with eight rats in each group.Allen's method was used to make T10 incomplete SCI model.Seven days after SCI,SCI-Ex group was given body weight-supported treadmill training.Before SCI,and seven days,14 days,21 days,28 days and 35 days after SCI,the von Frey filaments and thermal stimulation pain tester were used to evaluate the mechanical and thermal pain thresholds.Then,Western blotting and immunohistochemical analysis were performed on the spinal cord of all rats to detect the expression of GAD-65 and GAD-67.Results The mechanical and thermal pain thresholds were higher in SCI-Ex group than in SCI-Sed group 21 days,28 days and 35 days after SCI(P<0.01).Compared with the sham group,the expression of GAD-65 and GAD-67 decreased in SCI and SCI-Ex groups(P<0.05),and increased in SCI-Ex group compared with that of SCI-Sed group(P<0.05).Conclusion Body weight-supported treadmill training could increase the synthesis of GAD-65/67 in the distal spinal cord dorsal horn of incomplete SCI rats,and improve the pain thresholds of hind limbs in rats with SCI.

关 键 词：脊髓损伤 减重平板训练 神经病理性疼痛 Γ-氨基丁酸 谷氨酸脱羧酶-65/67 大鼠 

分 类 号：R651.2[医药卫生—外科学]