负载抗结核药物骨水泥生物力学实验研究  

A biomechanical analysis of anti-tuberculosis drug-loaded bone cement

作　　者：余涌杰[1] 赖震[1] 费骏[1] 胡胜平[1] YU Yong-jie;Lai Zhen;Fei Jun;HU Sheng-pin(Department of Orthopedics,Hangzhou Red-Cross hospital,Hangzhou 310003,Zhejiang,China)

机构地区：[1]杭州市红十字会医院骨科,杭州310003

出　　处：《浙江中西医结合杂志》2021年第2期121-124,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：杭州市科技发展计划项目(No.20150733Q41)。

摘　　要：目的观察负载抗结核药物对骨水泥材料生物力学性能的影响。方法无载药的骨水泥作为对照组,将40g骨水泥分别与不同剂量的利福平、异烟肼、乙胺丁醇、吡嗪酰胺,在无菌条件下真空搅拌,并加入骨水泥单体15mL,制成直径6mm、厚度3mm的矮圆柱体实验样品,分为R1组予负载0.75g利福平,R2组予负载1.5g利福平,R3组予负载2.25g利福平,H1组予负载0.3g异烟肼,H2组予负载0.6g异烟肼,H3组予负载0.9g异烟肼,E1组予负载0.75g乙胺丁醇,E2组予负载1.5g乙胺丁醇,E3组予负载2.25g乙胺丁醇,Z1组予负载1.5g吡嗪酰胺,Z2组予负载3.0g吡嗪酰胺,Z3组予负载4.5g吡嗪酰胺,每组6个,共制成78个试验样品。测定各骨水泥的面团时间和凝固时间。测量载抗结核药骨水泥的弯曲强度、弯曲模量和压缩强度的变化。结果载药骨水泥的压缩强度较标准骨水泥均有不同程度的降低,随着载药量越大压缩强度降低越明显。与对照组比较,低剂量载药组(R1组、H1组、E1组、Z1组)和中剂量载药组(R2组、H2组、E2组、Z2组)压缩强度差异无统计学意义(P>0.05),高剂量载药组(R3组、H3组、E3组、Z3组)压缩强度降低[(46.15±9.34)Mpa、(79.22±9.07)Mpa、(21.96±6.85)Mpa、(42.02±5.23)Mpa比(86.34±9.24)Mpa],差异有统计学意义(P<0.05)。载药骨水泥的弯曲强度和弯曲模量较对照组标准骨水泥均有不同程度的降低,随着载药量越大弯曲强度和弯曲模量降低越明显。与对照组比较,低剂量载药组(R1组、H1组、E1组、Z1组)弯曲强度和弯曲模量,差异无统计学意义(P>0.05),中剂量载药组(R2组、H2组、E2组、Z2组)和高剂量载药组(R3组、H3组、E3组、Z3组)弯曲强度[(46.87±3.72)Mpa、(38.38±3.51)Mpa、(28.38±2.75)Mpa、(36.31±2.38)Mpa比(60.86±5.30)Mpa,(26.89±2.36)Mpa、(21.70±4.47)Mpa、(19.79±2.03)Mpa、(22.38±1.79)Mpa比(60.86±5.30)Mpa]和弯曲模量[(1719.13±142.36)Mpa、(1482.20±93.50)Mpa、(1235.25±100.74)Mpa、(8Objective To investigate the effect of anti-tuberculosis drugs on the biomechanical properties of bone cement materials.Methods Bone cement without drug-loading was used as the control group.For experimental groups,40g of bone cement was mixed with different doses of rifampicin,isoniazid,ethambutol and pyrazinamide in vacuum under sterile conditions,and 15ml of bone cement monomer was added to make short cylinder experimental samples with a diameter of 6mm and a thickness of 3mm.After that,the samples were divided into R1(loaded with 0.75g rifampicin),R2(loaded with 1.5g rifampicin),R3(loaded with 2.25g rifampicin),H1(loaded with 0.3g isoniazid),H2(loaded 0.6g isoniazid),H3(loaded with 0.9g isoniazid),E1(loaded with 0.75g ethambutol),E2(loaded with 1.5g ethambutol),E3(loaded with 2.25g ethambutol),Z1(loaded with 1.5g pyrazinamide),Z2(loaded with 3.0g pyrazinamide),and Z3 groups(loaded with 4.5g pyrazinamide)(n=6 in each group).The dough time and setting time of bone cement were measured accordingly.The flexural strength,flexural modulus and compressive strength of bone cement in these different groups were also measured accordingly.Results Compared with the standard bone cement,the compressive strength of the drug-loaded bone cement was decreased in different degrees,and the compression strength was also decreased more obviously with the increase in drug loading.However,the compression strength showed no significant difference between the low-dose drug loading groups(R1,H1,E1,or Z1 group)and the medium-dose drug loading groups(R2,H2,E2,or Z2 group)vs.that of the control group(P>0.05),whereas it was significantly lower in the high-dose drug loading groups than that in the control group[(46.15±9.34)Pa in the R3 group,(79.22±9.07)Pa in the H3 group,(21.96±6.85)Pa in the E3 group,or(42.02±5.23)Pa in the Z3 group vs.(86.34±9.24)MPa in the control group;all(P<0.05)].Furthermore,the flexural strength and flexural modulus of the drug-loaded bone cement were decreased to varying degrees compared with those of the sta

关 键 词：抗结核药 骨水泥 生物力学 

分 类 号：R318.01[医药卫生—生物医学工程] R943[医药卫生—基础医学]