Bacterial community response to petroleum contamination in brackish tidal marsh sediments in the Yangtze River Estuary, China  

作　　者：Min Wang Chenyan Sha Jian Wu Jinghua Su Jianqiang Wu Qing Wang Juan Tan Shenfa Huang 

机构地区：[1]Research Institute of Natural Ecology Conseruation,Shanghai Academy of Enuvironmental Sciences,508 Qinzhou Road,Shanghai 200233,China

出　　处：《Journal of Environmental Sciences》2021年第1期160-167,共8页环境科学学报（英文版）

摘　　要：The brackish tidal marsh in the Baimaosha area of the Yangtze River Estuary was severely contaminated by 400 tons of heavy crude petroleum from a tanker that sank in December 2012.The spill accident led to severe environmental damage owing to its high toxicity,persistence and wide distribution.Microbial communities play vital roles in petroleum degradation in marsh sediments.Therefore,taxonomic analysis,high-throughput sequencing and 16 S rRNA functional prediction were used to analyze the structure and function of microbial communities among uncontaminated(CK),lightly polluted(LP),heavily polluted(HP),and treated(TD)sediments.The bacterial communities responded with increased richness and decreased diversity when exposed to petroleum contamination.The dominant class changed from Deltaproteobacteria to Gammaproteobacteria after petroleum contamination.The phylum Firmicutes increased dramatically in oil-enriched sediment by 75.78%,346.19%and 267.26%in LP,HP and TD,respectively.One of the suspected oil-degrading genera,Dechloromonas,increased the most in oil-contaminated sediment,by 540.54%,711.27%and 656.78%in LP,HP and TD,respectively.Spore protease,quinate dehydrogenase(quinone)and glutathione-independent formaldehyde dehydrogenase,three types of identified enzymes,increased enormously with the increasing petroleum concentration.In conclusion,petroleum contamination altered the community composition and microorganism structure,and promoted some bacteria to produce the corresponding degrading enzymes.Additionally,the suspected petroleum-degrading genera should be considered when restoring oil-contaminated sediment.

关 键 词：Brackish marsh sediment Petroleum contamination Bacterial community Gene enzyme 

分 类 号：X52[环境科学与工程—环境工程] X172