Restoring the plant productivity of heavy metal-contaminated soil using phosphate sludge,marble waste,and beneficial microorganisms  

作　　者：Anas Raklami Abdel-ilah Tahiri Noura Bechtaoui ElGharmali Abdelhay Eloisa Pajuelo Marouane Baslam Abdelilah Meddich Khalid Oufdou 

机构地区：[1]Laboratory of Microbial Biotechnologies,Agrosciences,and Enuironment(BioMAgE),Faculty of Sciences Semlalia,Cadi Ayyad University,Marrakech,Morocco [2]Laboratory of Agri-Food,Biotechnologies,and Valorization of Plant Bioresources(Agrobioval),Faculty of Sciences Semlalia,Cadi Ayyad University,Marrakech,Morocco [3]Laboratory of Water,Biodiversity and Global Change,Faculty of Sciences Semlalia,Cadi Ayyad University,Marrakech,Morocco [4]Department of Microbiology and Parasitology,University of Seville,41080 Seville,Spain [5]Laboratory of Biochemistry,Faculty of Agriculture,Niigata University,Niigata 950-2181,Japan [6]Department of Life and Food Sciences,Graduate School of Science and Technology,Nigata University,Niigata 950-2181,Japan

出　　处：《Journal of Environmental Sciences》2021年第1期210-221,共12页环境科学学报（英文版）

基　　金：supported by PPR2/2016/42 project(CNRST Morocco);KAKENHI Grants-in-Aid for Scientific Research(A)(15H02486)from Japan Society for the Promotion of Sciences;Strategic International Collaborative Research Program by the Japan Science and Technology Agency(JPMJSC16C5);Grant for Promotion of KAAB Projects(Niigata University)from the Ministry of Education,Culture,Sports,Science,and Technology,Japan。

摘　　要：Assisted natural remediation(ANR)has been highlighted as a promising,less expensive,and environmentally friendly solution to remediate soil contaminated with heavy metals.We tested the effects of three amendments(10%compost,C;5 or 15%phosphate sludge,PS5 and PS15;and 5 or 15%marble waste,MW5 and MW15)in combination with microorganism inoculation(rhizobacteria consortium alone,mycorrhizae alone,and the two incombination)on alfalfa in contaminated soil.Plant concentrations of Zn,Cu,and Pb were measured,along with proline and malondialdehyde production.The microbiological and physicochemical properties of the mining soil were evaluated.Application of the amendments allowed germination and promoted growth.Inoculation with the rhizobacteria consortium and/or mycorrhizae stimulated plant growth.PS and MW stimulated the production of proline.Inoculation of alfalfa with the rhizobacteria-mycorrhizae mixture and the application of MW allowed the safe cultivation of the legume,as shown by the low concentrations of metals in plant shoots.Zn and Pb concentrations were below the limits recommended for animal grazing and accumulated essentially in roots.Soil analyses showed the positive effect of the amendments on the soil physicochemical properties.All treatments increased soil p H(around 7),total organic carbon,and assimilable phosphorus content.Notably,an important decrease in soluble heavy metals concentrations was observed.Overall,our findings revealed that the applied treatments reduced the risk of metal-polluted soils limiting plant growth.The ANR has great potential for success in the restoration of polymetallic and acidic mining soils using the interaction between alfalfa,microorganisms,and organomineral amendments.

关 键 词：BIOAUGMENTATION BIOREMEDIATION Mine soil Heavy metals Immobilization Rhizostabilization Root microbiome Wastes added-value 

分 类 号：X53[环境科学与工程—环境工程]