PolyI:C刺激豚鹿(Axis porcinus)外周血淋巴细胞转录组分析  

Transcriptome Analysis of Hog Deer(Axis Porcinus)Peripheral Blood Lymphocyte after PolyI:C Challenge

作　　者：隋维恺 高艳 杨茜茜 冷思竹 严慧娟[2] 屈羽[2] 陈维刚[2] 杜小刚[1] SUI Weikai;GAO Yanfeng;YANG Xixi;LENG Sizhu;YAN Huijuan;QU Yu;CHEN Weigang;DU Xiaogang(College of Life Sciences,Sichuan Agricultural University,Ya'an 625014,Sichuan,China;ChengduWildlife Research Institute,Chengdu Zoo,Chengdu 610081,China)

机构地区：[1]四川农业大学生命科学学院,四川雅安625014 [2]成都动物园成都市野生动物研究所,成都610081

出　　处：《四川农业大学学报》2021年第1期114-120,128,共8页Journal of Sichuan Agricultural University

基　　金：成都动物园项目基金(2019072)。

摘　　要：【目的】初步探究豚鹿的抗病毒免疫机制,为野生动物保护提供新的思路。【方法】利用转录组测序技术对聚肌胞苷酸(PolyI:C)刺激后的豚鹿外周血淋巴细胞进行测序分析并使用qPCR方法对数据的准确性进行验证。【结果】拼接得到121204条unigene,平均长度1121 bp。共有97.12%的unigene得到成功注释。经过差异表达分析,获得402个差异表达基因(DEGs)。GO和KEGG富集分析显示差异基因在免疫反应方面出现富集,进而筛选出28个免疫相关基因。随机选取9个基因进行实时荧光定量分析,验证结果与测序结果变化趋势一致。【结论】获得了Poly I:C刺激后豚鹿外周血淋巴细胞的基因表达谱,为豚鹿抗病毒免疫应答机制的研究奠定了基础,为疫苗佐剂的开发及应用提供了理论基础。【Objective】The purpose of this study is to initially explore the antiviral immune mechanism of hog deer and provide new ideas for wildlife protection.【Method】RNA-seq was used to analyze the peripheral blood lymphocytes of hog deer after poly I:C stimulation and the accuracy of the data was ver ified by qPCR.【Result】121204 unigenes were spliced,with an average length was 1121 bp.After functional annotations,97.12%unigenes were successfully annotated.Through DEG-seq analysis,402 DEGs were obtained.The DEGs were subjected to enrichment analysis of GO and KEGG,and then 28 immune-related genes were screened out.9 immune-related genes were selected for qPCR verification,and the results were consistent with the trend of sequencing.【Conclusion】This study obtained the gene expression profile after Poly I:C stimulation,which laid the basic data for the study of the immune re sponse mechanism in the antiviral defense of hog deer,and provided a theoretical basis for the develop ment and application of vaccine adjuvants.

关 键 词：Poly I:C 豚鹿 外周血淋巴细胞(PBL) 转录组 qPCR 

分 类 号：Q952[生物学—动物学]