miR-130b-3p靶向肝细胞生长因子调控妊娠滋养层细胞增殖、迁移和侵袭的机制研究  

Mechanism of miR-130b-3p targeting hepatocyte growth factor in regulating proliferation,migration and invasion of gestational trophoblast cells

作　　者：刘韵 邢辉 兰艳丽 LIU Yun;XING Hui;LAN Yan-li(Department of Obstetrics and Gynecology,Xiangyang Central Hospital,Affiliated Hospital of Hubei University of Arts and Science,Xiangyang 441021,Hubei Province,China)

机构地区：[1]湖北文理学院附属医院襄阳市中心医院妇产科,湖北襄阳441021

出　　处：《中国临床药理学杂志》2021年第1期36-40,共5页The Chinese Journal of Clinical Pharmacology

基　　金：湖北省自然科学基金资助项目(WJ2015MA024);襄阳市科技局基金资助项目(襄科业[2013]68号-10)。

摘　　要：目的研究miR-130b-3p靶向肝细胞生长因子(HGF)调控妊娠滋养层细胞增殖、迁移、侵袭的分子机制。方法以实时荧光定量逆转录聚合酶链反应检测正常妊娠妇女胎盘组织和妊娠期子痫前期患者胎盘组织中miR-130b-3p的表达。分别建立抑制miR-130b-3p表达和过表达HGF的JEG-3细胞株,用噻唑蓝法检测细胞活力;用Transwell法检测细胞的迁移及侵袭能力;以蛋白质印迹法检测HGF蛋白的表达,用双荧光素酶报告基因实验验证miR-130b-3p对HGF的靶向作用。结果与正常妊娠妇女胎盘组织(0.29±0.01)相比,妊娠期子痫前期患者胎盘组织(0.60±0.03)中miR-130b-3p的表达显著升高(P<0.05)。anti-miR-NC组和anti-miR-130b-3p组滋养层细胞JEG-3的迁移数分别为85.67±8.67,130.67±12.67,侵袭细胞数分别为74.33±4.66,115.67±12.67,差异有统计学意义(P<0.05)。pcDNA3.1组和pcDNA3.1-HGF组迁移数分别为81.50±12.50,116.00±18.00,侵袭数分别为75.00±8.00,94.50±12.50,差异均有统计学意义(均P<0.05)。anti-miR-130b-3p+si-NC组和anti-miR-130b-3p+si-HGF组迁移细胞数分别为131.67±10.67,108.00±6.00,侵袭细胞数分别为117.30±18.67,96.33±8.67,差异有统计学意义(均P<0.05)。结论miR-130b-3p通过靶向下调HGF可抑制妊娠滋养层细胞的增殖、迁移和侵袭。Objective To investigate the molecular mechanism of miR-130 b-3 p targeting hepatocyte growth factor(HGF)in regulating the proliferation,migration and invasion of gestational trophoblast cells.Methods Real-time fluorescence quantitative reverse transcription polymerase chain reaction was used to detect the expression of miR-130 b-3 p in placental tissues of normal pregnant women and placental tissues of preeclampsia patients during pregnancy.JEG-3 cell lines which inhibited the expression of miR-130 b-3 p or over-expressed HGF were established,and the cell viability was detected by methyl thiazolyl tetrazolium assay,the migration and invasion ability of cells were detected by Transwell assay,the expression of HGF was detected by Western blot.The dual luciferase reporter gene assay was used to verify the targeting of miR-130 b-3 p to HGF.Results Compared with placental tissue in normal pregnant women(0.29±0.01),the expression of miR-130 b-3 p(0.60±0.03)in placental tissues of preeclamptic patients during pregnancy was significantly increased(P<0.05).The migrate ability of JEG-3 cells in anti-miR-NC group and anti-miR-130 b-3 p group were 85.67±8.67,130.67±12.67,the invade ability were 74.33±4.66,115.67±12.67,with significant difference(P<0.05).The migrate ability in pcDNA3.1 group and pcDNA3.1-HGF group were 81.50±12.50,116.00±18.00,the invade ability were 75.00±8.00,94.50±12.50,with significant difference(P<0.05).The migrate ability in anti-miR-130 b-3 p+si-NC group and anti-miR-130 b-3 p+si-HGF group were131.67±10.67,108.00±6.00,the invade ability 117.30±18.67,96.33±8.67,all with significant difference(all P<0.05).Conclusion miR-130 b-3 p can inhibit the proliferation,migration and invasion of gestational trophoblast cells by targeting down-regulation of HGF.

关 键 词：肝细胞生长因子 子痫前期 细胞增殖 迁移 侵袭 

分 类 号：R979.1[医药卫生—药品]