Metabolomics study of biochemical changes in the serum and articular synovium tissue of moxibustion in rats with collagen-induced arthritis  

艾灸对胶原诱导性关节炎大鼠血清和关节滑膜组织生化改变的代谢组学研究

作　　者：Xiang-tian PANG Yong-yi ZHANG Yu-fei LENG Yao YAO Rui Zhang Dan-wen WANG Xiao XU Zhi-ling SUN 庞祥天;张永怡;冷雨飞;姚瑶;张睿;王丹文;徐骁;孙志岭(南京中医药大学护理学院,中国南京210023;南京卫生高等职业技术学校护理系;浙江中医药大学护理学院,中国杭州310053)

机构地区：[1]School of Nursing,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China [2]Nursing Department,Nanjing Vocational Health College,Nanjing,Jiangsu Province,China [3]School of Nursing,Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang Province,China

出　　处：《World Journal of Acupuncture-Moxibustion》2021年第1期30-43,共14页世界针灸杂志（英文版）

基　　金：National Natural Science Foundation of China:81774383,81904274;supported by"Nursing Advantageous Discipline Construction Project in Jiangsu Universities"of Nanjing University of Chinese medicine:2019YSHL008,2019YSHL021。

摘　　要：Objective:To demonstrate the rheumatoid arthritis(RA)mechanisms by determining the biochemical changes.To investigate the therapeutic mechanism of moxibustion in RA-model rats using a gas chromatography-mass spectrometry(GC-MS)metabolomics approach.Methods:A total of 24 rats were divided into three groups as follows:normal control group,model group and moxibustion group.Rats in model group and moxibustion group were set up collagen-induced arthritis(CIA)model.Rats in moxibustion group were treated by moxibustion.After 3 weeks of intervention,right ankle joint,serum and articular synovium samples were collected.Right ankle joint samples were used for histopathological evaluation between 3 groups to get the pathological changes of tissues and cells.Serum and articular synovium samples were used to analyze the changed metabolites of moxibustion on RA rats by the GC-MS based metabolomics.Results:Treatment of moxibustion not only significantly increased the weight of CIA rats,reduced the swelling of hind paw,arthritic scores,IL-1β,TNF-αbut also improved histopathological evaluation in right ankle joint samples.Sixteen significantly altered metabolites were found in RA rats as potential biomarkers of arthritis.Thirteen metabolites,significantly adjusted by moxibustion to help relieve arthritis,were selected out as biomarkers of antiarthritic mechanism of moxibustion,which were mainly involved in phenylalanine,tyrosine and tryptophan biosynthesis,glycine,serine and threonine metabolism,phenylalanine metabolism,alanine,aspartate and glutamate metabolism,glyoxylate and dicarboxylate metabolism and aminoacyl-tRNA biosynthesis.Conclusions:We have indicated moxibustion treatment is able to resist inflammation in CIA rats effectively.Using GC-MS metabolomics technique,we detect novel metabolites in the moxibustion antiarthritic process,which may aid in advanced understanding of arthritis and therapeutic mechanism of moxibustion.

关 键 词：Gas chromatography-mass spectrometry Metabolomics MOXIBUSTION Rheumatoid arthritis Collagen-induced arthritis 

分 类 号：R245.8[医药卫生—针灸推拿学]