地克珠利纳米乳含量测定方法的建立及稳定性研究  

Establishment and Stability of Determination Method for Content of Dikzuli Nano-emulsion

作　　者：丁宁[1] 洪伟鸣[1] 张梁秋 DING Ning;HONG Wei-ming;ZHANG Liang-qiu(Jiangsu Agri-animal Husbandry Vocational College,Jiangsu Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院,江苏泰州225300

出　　处：《广州化工》2021年第4期69-71,74,共4页GuangZhou Chemical Industry

基　　金：江苏农牧科技职业学院大学生创新创业训练计划校级创新项目(No:201912806080Y)。

摘　　要：建立高效液相色谱法测定地克珠利纳米乳中地克珠利含量的方法并对其稳定性进行研究,采用Waters Xbridge C 18(150 mm×4.6 mm,5μm)色谱柱,以乙腈:水:磷酸:冰乙酸(54:46:0.15:0.15,V/V)为流动相,流速1 mL/min,检测波长280 nm,进样量20μL,柱温25℃。结果显示:地克珠利纳米乳在该色谱条件下,系统适用性良好,在2.02~40.48μg/mL的浓度范围内线性关系良好。地克珠利纳米乳在温度为40℃、相对湿度(RH)为75%的条件下稳定。In order to establish a high performance liquid chromatography(HPLC)method for the determination of diclazuril in diclazuril nano-emulsion and study its stability,Waters Xbridge C 18(150 mm×4.6 mm,5μm)column was used,using acetonitrile:water:phosphate:acetic acid(54:46:0.15,V/V)as mobile phase,flow rate of 1 mL/min,detection wavelength of 280 nm,injection volume of 20μL,column temperature of 25℃.The stability of diclazuril nano-emulsion was investigated by response factor test and accelerated test.The results showed that the system had good applicability under this chromatographic condition,and the linear relationship was good in the concentration range of 2.02~40.48μg/mL.Diclazuril nano-emulsion was stable at 40℃and 75%relative humidity(RH).

关 键 词：地克珠利 纳米乳 高效液相色谱法 稳定性 

分 类 号：S859.79[农业科学—临床兽医学]