3D打印不同孔径钛合金支架修复兔股骨缺损:600μm孔径更有利于骨整合  

Three-dimensional printed porous titanium alloy scaffolds with different apertures in repair of femoral defects in rabbits:600μm aperture is more conducive to osseointegration

作　　者：王庆 翁益平 刘宏伟 张文[3] 施勤[3] 张润泽 蒋俊锋 王彩梅 Wang Qing;Weng Yiping;Liu Hongwei;Zhang Wen;Shi Qin;Zhang Runze;Jiang Junfeng;Wang Caimei(Graduate School,Dalian Medical University,Dalian 116044,Liaoning Province,China;Department of Orthopedics,Changzhou Second People’s Hospital Affiliated to Nanjing Medical University,Changzhou 213003,Jiangsu Province,China;Institute of Orthopedics,Soochow University,Suzhou 215006,Jiangsu Province,China;School of Internet of Things Engineering,Hehai University,Changzhou 213022,Jiangsu Province,China;Beijing Aikang Yicheng Co.,Ltd.,Beijing 102200,China)

机构地区：[1]大连医科大学研究生院,辽宁省大连市116044 [2]南京医科大学附属常州第二人民医院骨科,江苏省常州市213003 [3]苏州大学骨科研究所,江苏省苏州市215006 [4]河海大学物联网工程学院,江苏省常州市213022 [5]北京爱康宜诚股份有限公司,北京市102200

出　　处：《中国组织工程研究》2021年第28期4441-4446,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学面上基金资助项目(61772172),项目参与者:蒋俊锋、刘宏伟;常州市卫计委重大项目资助(ZD201617),项目负责人:刘宏伟。

摘　　要：背景:多孔钛合金的微孔结构不但能为成骨细胞的增殖与迁移提供充分的空间,而且能增强间充质干细胞的成血管作用,影响成骨基因的表达和成骨细胞分化,是骨整合的重要因素。目的:分析不同孔径3D打印多孔钛合金支架修复兔股骨外髁骨缺损的骨整合能力。方法:将20只新西兰兔40条后腿随机分为A、B、C、D组,制备股骨外髁5 mm×5 mm骨缺损模型,A、B、C组分别植入200,600,1000μm孔径多孔钛合金支架,D组不植入材料。术后4,8周拍摄X射线片,观察植入物有无移位、脱出及植入物周围是否存在骨溶解、骨坏死等现象;术后8周时,Micro-CT检测股骨标本骨小梁厚度、骨小梁数量、骨小梁相对体积、骨表面积密度、骨小梁分离度,组织学观察骨整合情况。实验方案经苏州大学动物实验伦理委员会批准。结果与结论:①X射线片显示,内植物位置良好,无明显移位和脱落,材料周围骨形成良好,未见骨溶解、骨坏死等现象;②Micro-CT检测显示,3组植入物的孔隙内及植入物周围均不同程度地有骨组织存在,B组微孔的骨小梁数量及骨表面积密度高于A、C组(P<0.05),A组微孔的骨小梁厚度大于B、C组(P<0.05);③甲苯胺蓝染色显示,B组几乎所有表层微孔内均有新生骨组织长入,部分新生骨延伸进入支架的深层孔隙中,与多孔结构结合紧密,形成机械交锁;A、C组仅有少量表层微孔存在新生骨组织长入,深层孔隙无明显的新生骨质形成,新生骨与植入物结合较差;④结果表明相对于200,1000μm孔径,600μm孔径3D打印多孔钛合金支架更有利于新骨形成。BACKGROUND:The microporous structure of a porous titanium alloy can not only provide sufficient space for the proliferation and migration of osteoblasts,but also promote the angiogenesis of mesenchymal stem cells,affect the expression of osteogenic genes and the differentiation of osteoblasts,which is an important factor for bone integration.OBJECTIVE:To analyze the osseointegration ability of three-dimensional printed porous titanium alloy scaffolds with different apertures in repairing rabbit femoral condyle defect.METHODS:Totally 40 hind legs of 20 New Zealand rabbits were randomly divided into group A,group B,group C,and group D.A 5 mm×5 mm bone defect model was established.Groups A,B,and C were implanted with porous titanium alloy scaffolds with a diameter of 200,600,and 1000μm,while group D was not implanted.X-ray films were taken at 4 and 8 weeks after operation to observe whether there was displacement,prolapse and osteolysis,and osteonecrosis around the implants.At 8 weeks after operation,the trabecular thickness,trabecular number,trabecular volume,bone surface area density,and trabecular separation were detected by micro CT.The osseointegration was observed by histology.The experimental protocol was approved by the Animal Experiment Ethics Committee of Soochow University.RESULTS AND CONCLUSION:(1)X-ray films showed that the implants were in good position,without obvious displacement or falling off,and the bone around the materials was good,without osteolysis,osteonecrosis or other bad phenomena.(2)Micro-CT examination showed that bone tissue existed in the pores and around the implants in three groups.The number of trabeculae and bone surface area density in group B were significantly higher than those in the groups A and C(P<0.05).The trabecular thickness of micropores in the group A was significantly higher than that in the groups B and C(P<0.05).(3)Toluidine blue staining showed that the new bone tissue grew into almost all the surface micropores in group B,and some of the new bone extended into th

关 键 词：骨 材料 孔径 多孔 钛合金 新骨形成 3D打印 电子束熔融技术 骨缺损 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]