脱细胞脱钙骨基质-促红细胞生成素水凝胶促成骨和成血管的能力  

A hydrogel based on acellular decalcified bone matrix/erythropoietin promotes osteogenesis and angiogenesis

作　　者：周建伟[1] 周静[2] 李矛 迟成 王飞[1] Zhou Jianwei;Zhou Jing;Li Mao;Chi Cheng;Wang Fei(Department of Orthopedics,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Department of Joint Surgery,Tengzhou Central People’s Hospital,Jining Medical College,Tengzhou 277500,Shandong Province,China)

机构地区：[1]首都医科大学附属北京同仁医院骨科,北京市100730 [2]济宁医学院附属滕州市中心人民医院关节外科,山东省滕州市277500

出　　处：《中国组织工程研究》2021年第28期4454-4459,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:近年来脱细胞脱钙骨基质(acellular decalcified bone matrix,ADBM)因其良好的生物相容性和生物力学性能在骨缺损修复中有着巨大潜力和应用前景,而血管生成是骨修复中的重要环节,促红细胞生成素(erythropoietin,EPO)可促进局部血管的形成从而间接调节骨修复。目的:观察负载EPO的ADBM(ADBM-EPO)水凝胶的促成骨和成血管能力。方法:构建ADBM水凝胶与ADBM-EPO水凝胶。将人骨髓间充质干细胞与人脐静脉内皮细胞分别接种于两种水凝胶上,以常规培养的细胞为对照组,通过细胞跨膜侵袭及细胞划痕迁移实验检测水凝胶对两种细胞迁移能力的影响,通过碱性磷酸酶及茜素红染色检测两种水凝胶对骨髓间充质干细胞成骨分化的影响。结果与结论:①与对照组比较,ADBM水凝胶与ADBM-EPO水凝胶可以促进骨髓间充质干细胞或脐静脉内皮细胞的跨膜迁移,其中ADBMEPO水凝胶的促进作用更显著;②与对照组比较,ADBM水凝胶与ADBM-EPO水凝胶可以缩小人骨髓间充质干细胞或人脐静脉内皮细胞的划痕距离,其中ADBM-EPO水凝胶的作用更显著;③与对照组比较,ADBM水凝胶与ADBM-EPO水凝胶可提高骨髓间充质干细胞的碱性磷酸酶活性与钙结节数量,其中ADBM-EPO水凝胶的作用更显著;④结果表明,ADBM-EPO水凝胶具有促成骨与促血管形成的双向作用。BACKGROUND:In recent years,acellular decalcified bone matrix(ADBM)exhibits tremendous potential and application prospects in bone defect repair due to its favorable biocompatibility and biomechanics.Erythropoietin(EPO)can promote the formation of local blood vessels and thus indirectly regulate bone repair owing to crucial role in angiogenesis of bone repair.OBJECTIVE:To investigate the effects of acellular decalcified bone matrix/erythropoietin(ADBM-EPO)hydrogel on osteogenesis and angiogenesis.METHODS:ADBM hydrogel and ADBM-EPO hydrogel were constructed.Human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells were respectively seeded on the two hydrogels,and the conventionally cultured cells were used as the control group.The effects on the migration ability of two kinds of cells were detected through cell transmembrane invasion and cell scratch migration experiments.The effects of the two hydrogels on osteogenic differentiation of bone marrow mesenchymal stem cells were determined by alkaline phosphatase and alizarin red staining.RESULTS AND CONCLUSION:(1)Compared with the control group,ADBM hydrogel and ADBM-EPO hydrogel could promote the migration of bone marrow mesenchymal stem cells or umbilical vein endothelial cells.Moreover,the ADBM-EPO hydrogel had better migration ability than that of ADBM hydrogel.(2)Compared with the control group,both ADBM and ADBM-EPO hydrogels could reduce the scratch distance of human bone marrow mesenchymal stem cells or human umbilical vein endothelial cells.Of them,ADBM-EPO hydrogel was more effective than ADBM hydrogel.(3)Compared with the control group,both ADBM and ADBM-EPO hydrogels could increase the alkaline phosphatase activity and the number of calcium nodules in bone marrow mesenchymal stem cells.Moreover,the ADBM-EPO hydrogel had better ability than that of ADBM hydrogel.(4)The above experimental results showed that the ADBM-EPO hydrogel had a bidirectional role in promoting osteogenesis and angiogenesis.

关 键 词：材料 水凝胶 脱细胞 脱钙骨基质 促红细胞生成素 细胞迁移 成骨分化 干细胞 内皮细胞 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]