Highly sensitive detection of phosphopeptides with superparamagnetic Fe_(3)O_(4)@mZrO_(2) core-shell microspheres-assisted mass spectrometry  

作　　者：Ruifang Gao Jin Li Rui Shi Yang Zhang Fuzhou Ouyang Ting Zhang Lihua Hu Guoqiang Xu Jian Liu 

机构地区：[1]Institute of Functional Nano&Soft Materials(FUNSOM),Jiangsu Key Laboratory for Carbon-Based Functional Materials&Devices,Soochow University,Suzhou 215213,China [2]Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences Soochow University,Suzhou 215213,China

出　　处：《Journal of Materials Science & Technology》2020年第24期234-242,共9页材料科学技术（英文版）

基　　金：supported by the National Key Research and Development Program of China(2017YFE0131700);and the National Natural Science Foundation of China(21874096);a project supported by Collaborative Innovation Center of Suzhou Nano Science and Technology;the 111 Project;Joint International Research Laboratory of Carbon-Based Functional Materials and Devices;the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：Protein phosphorylation is one of the most important post-translational modifications.It is an active research area to study phosphoproteomics for discovery of disease biomarkers and druggable targets.Here we report the development of superparamagnetic Fe_(3)O_(4)@mZrO_(2) core-shell microspheres with mesoporous structures for highly efficient enrichment of phosphopeptides.We have demonstrated that the mesoporous ZrO_(2) layer dramatically improves the selective enrichment of phosphopeptides.Our approach allows for in-situ elution and sensitive identification of both mono-phosphorylated and multiphosphorylated peptides in MALDI-TOF mass spectrometry,with the detection limit of down to the femtomole range.The target phosphopeptides can reliably be enriched for MS analysis from various complex samples including the spiked protein digests and tumor cell lysates.The Fe_(3)O_(4)@mZrO_(2) coreshell microspheres promise a useful tool for phosphoproteomics by allowing for highly efficient and selective enrichment of the crucial signaling regulators in a low abundance.

关 键 词：Core-shell microspheres Mesoporous structure Protein phosphorylation MALDI-TOF mass spectrometry Enrichment technologies 

分 类 号：R318[医药卫生—生物医学工程] O657.63[医药卫生—基础医学]