Comparison of benzothiazole-based dyes for sensitive DNA detection  

作　　者：Yanying Wang Ronghui Zhou Wenxue Liu Chao Liu Peng Wu 

机构地区：[1]College of Chemistry,Sichuan University,Chengdu 610064,China [2]Stare Key Laboratory of Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China [3]State Key Laboratory of Hydraulics a n d Mountain River Engineering,College of Water Resource&1 Hydropower,Sichuan University,Chengdu 610065,China

出　　处：《Chinese Chemical Letters》2020年第11期2950-2954,共5页中国化学快报（英文版）

基　　金：the Major Scientific and Technological Application Program of Chengdu(No.2019-YF09-0081-SN);the Major Scientific and Technological Special Program of Sichuan Province(No.2018SZDZX0027);the Fundamental Research Funds for Central Universities(No.2018SCUH0075)。

摘　　要：For efficient and quantitative DNA detection,fluorescence staining is the most often explored approach,which relies on non-covalent binding of dyes with double stranded DNA(dsDNA).Ethidium bromide(EB)is the most classic DNA stain,but suffers from its high carcinogenicity.A series of less toxic alternatives were developed,many of which contain the core structure of the benzothiazole ring.However,the relationship between the structure and the DNA detection performance was not illustrated.Herein,five benzothiazole dyes,namely thiazole orange,SYBR Green I,Pico Green,SYBR Safe,and thioflavine-T,were compared for DNA detection through direct fluorescence and gel electrophoresis,with particular focus on the structure-performance relationship.It turned out that SYBR Green I is currently the best choice for DNA detection.The results in this work may be useful for future DNA-staining dye developments.

关 键 词：DNA quantitative detection BENZOTHIAZOLE SYBR Green I Gel electrophoresis 

分 类 号：Q503[生物学—生物化学] O657.3[理学—分析化学]