A general strategy to quantify analytes through fluorescence chromaticity and luminosity  

作　　者：Tianyu Liang Peiwei Yang Tianhong Wu Menghan Shi Xiayu Xu Taotao Qiang Xiaolong Sun 

机构地区：[1]The Key Laboratory of Biomedical Information Engineering of Ministry of Education,School of Life Science and Technology,Xi'an Jiaotong University,Xi'an 710049,China [2]College of Bioresources and Materials Engineering,Shaanxi University of Science,Technology,Xi'an 710021,China [3]Bioinspired Engineering and Biomechanics Center(BEBC),Xi'an Jiaotong University,Xi'an 710049,China

出　　处：《Chinese Chemical Letters》2020年第11期2975-2979,共5页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(No.21907080);Natural Science Foundationof Shaanxi(No.2020JM-069);Key Scientific Research Group of Shaanxi Province(No.2020TD-009);the Youth Innovation Team of Shaanxi Universities。

摘　　要：To realize a fast,easy-operation and precise way using fluorescence probes to quantify analytes is a goal to facilitate detection,especially in situ.Herein,we are reporting an approach which can be generally employed for the differentiation and quantitation of analytes through fluorescence chromaticity and luminosity.Seven representative fluorescent probes,targeting p H,cysteine,hydrogen sulfide,hydrogen peroxide,palladium and hydrazine,were synthesized and tested.Without utilizing costly instrumentations,portable devices were employed to collect data of photographs from the fluorescence samples in responses to different analytes.Subsequently,the photographic images were digitally processed to generate calibration curves between chromaticity/luminosity verse concentrations after mapping to the CIE 1931 xy Y standard color space.Good linear calibration curves and quantitative analysis of unknown samples with low errors through the spectral technology demonstrated the reliability of this method.Thus,we showed the analytical method with a simple and on-site constructible/portable device which is promising for applications in more fluorescence probes.

关 键 词：Fluorescence probes Portable device Calibration curve PHOTOGRAPHY Chromaticity and luminosity 

分 类 号：O657.3[理学—分析化学]