Molecular engineering of ultra-sensitive fluorescent probe with large Stokes shift for imaging of basal HOCl in tumor cells and tissues  

作　　者：Peng Lu Xingxing Zhang Tianbing Ren Lin Yuan 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China

出　　处：《Chinese Chemical Letters》2020年第11期2980-2984,共5页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.21877029,21735001);the National Key R&D Program of China(No.2019YFA0210103);the National Postdoctoral Program for Innovative Talents(No.BX20190110);the China Postdoctoral Science Foundation(No.2019M662758)。

摘　　要：Fluorescent probes have been widely employed in biological imaging and sensing.However,it is always a challenge to design probes with high sensitivity.In this work,based on rhodamine skeleton,we developed a general strategy to construct sensitivity-enhanced fluorescent probe with the help of theoretical calculation for the first time.As a proof of concept,we synthesized a series of HOCl probes.Experiment results showed that with the C-9 of pyronin moiety of rhodamine stabilized by an electron donor group,probe DQF-S exhibited an importantly enhanced sensitivity(LOD:0.2 nmol/L)towards HOCl together with fast response time(<10 s).Moreover,due to the breaking symmetrical electron distribution by another electron donor group,the novel rhodamine probe DQF-S displayed a far red to near-infrared emission(>650 nm)and large Stokes shift.Bioimaging studies indicated that DQF-S can not only effectively detect basal HOCl in various types of cells,but also be successfully applied to image tumor tissue in vivo.These results demonstrate the potential of our design as a useful strategy to develop excellent fluorescent probes for bioimaging.

关 键 词：Fluorescent probes High sensitivity Hypochlorous acid(HOCl) Large Stokes shift Fluorescence imaging 

分 类 号：Q503[生物学—生物化学] O657.3[理学—分析化学]