扶正清热通淋汤对多药耐药大肠埃希菌生物被膜大鼠模型的影响  

Effect of Fuzheng Qingre Tonglin Decoction on biofilm of multi-drug resistant Escherichia coli of rat model

作　　者：罗小全 粟宏伟 李兴宇 杜明月 朱永生 刘樊 刘鑫 LUO Xiao-quan;SU Hong-wei;LI Xing-yu;DU Ming-yue;ZHU Yong-sheng;LIU Fan;LIU Xin(Affiliated Hospital of Traditional Chinese Medicine of Southwest Medical University,Luzhuu,Sichuan 646000,China;不详)

机构地区：[1]西南医科大学附属中医医院泌尿外科,四川泸州646000 [2]成都市第八人民医院心血管内科,四川成都610000 [3]航空工业三六三医院泌尿外科,四川成都610000

出　　处：《中华医院感染学杂志》2020年第23期3584-3588,共5页Chinese Journal of Nosocomiology

基　　金：泸州市人民政府-西南医科大学科技战略合作项目(2017LZXNYD-T09);泸州市科技计划项目(2019-JYJ-67)。

摘　　要：目的探讨扶正清热通淋汤对多药耐药大肠埃希菌生物被膜的影响。方法通过结晶紫染色法测定18株临床分离多药耐药大肠埃希菌生物被膜形成能力,筛选出强成膜菌备用。经尿道置入自制环形硬膜外导管于大鼠膀胱内,并注入强成膜菌,建立大鼠体内多药耐药大肠埃希菌尿路感染生物被膜模型,随机将大鼠分为模型组,左氧氟沙星组,扶正清热通淋汤组,左氧氟沙星+扶正清热通淋汤组;并另设空白对照组。造模后第5天开始灌胃给药,连续给药14 d。取大鼠尿液进行细菌培养,对取出的各组膀胱内异物采用扫描电镜观察各组异物表面细菌形态及生物被膜结构情况,同时采用积分法定量比较各组生物被膜产生量。结果 18株多药耐药大肠埃希菌有13株产生物被膜,占比72.22%,其中强产膜菌3株、中产模菌4株、弱产膜菌6株;空白对照组尿培养结果阳性率为0、模型组100.00%、扶正清热通淋汤组为66.67%、左氧氟沙星组为66.67%、左氧氟沙星+扶正清热通淋汤组为37.50%;膀胱内异物生物被膜观察:扶正清热通淋汤组、左氧氟沙星+扶正清热通淋汤组同模型组生物被膜积分比较差异有统计学意义(P<0.05)。结论本实验显示临床分离多药耐药大肠埃希菌产生物被膜比例较高;扶正清热通淋汤具有清除细菌生物被膜的作用;扶正清热通淋汤联合左氧氟沙星对多药耐药大肠埃希菌生物被膜有较好的清除能力,并对被膜游离菌有杀灭作用。OBJECTIVE To explore the effect of Fuzheng Qingre Tonglin Decoction on the biofilm of multi-resistant Escherichia coli. METHODS The ability of biofilm formation in 18 clinical multi-drug resistant E coli strains was determined by crystal violet staining, and strong biofilm-forming bacteria were selected for use. A self-made circular epidural catheter was inserted into the bladder of the rat through the urethra, and strong biofilm-forming bacteria were injected to establish a biofilm model of multi-drug resistant E.coli urinary tract infection in rats. The rats were randomly divided into the model groups, levofloxacin group, Fuzheng Qingre Tonglin Decoction group, levofloxacin + Fuzheng Qingre Tonglin Decoction group and blank control group. On the 5 th day after the model was established, drugs were intragastric administrated for 14 days. The urine of the rats was collected for bacterial culture, and the bacterial morphology and biofilm structure on the surface of the foreign bodies in each group were observed by scanning electron microscopy. The amount of biofilm produced was quantitatively compared in all groups by the integration method. RESULTS There were 13 strains of multi-drug resistant E.coli producing membranes, accounting for 72.22%, which included 3 strains of strong biofilm-forming bacteria, 4 strains of medium biofilm-forming bacteria and 6 strains of weak biofilm-forming bacteria. The rate of urine positive bacterial culture in the blank control group were 0, and that in the model group, Fuzheng Qingre Tonglin Decoction group, levofloxacin group, levofloxacin + Fuzheng Qingre Tonglin Decoction group were 100.00%, 66.67%, 66.67% and 37.50%, respectively. Biofilm scores in the Fuzheng Qingre Tonglin Decoction group, levofloxacin + Fuzheng Qingre Tonglin Decoction group were significantly different from that in the model group(P<0.05). CONCLUSION This experiment showed that the proportion of clinically isolated multi-drug-resistant E.coli producing biofilm was relatively high, and Fuzheng Qingre Tongli

关 键 词：中药 扶正清热通淋汤 多药耐药大肠埃希菌 细菌生物被膜 

分 类 号：R285.5[医药卫生—中药学]