乙肝肝硬化患者血清HBV-DNA载量与HSP70、CEA及AFP水平的相关性  

Correlation between serum HBV-DNA load and levels of HSP70, CEA and AFP in patients with hepatitis B cirrhosis

作　　者：陈颖 方亮[1] 刘正爱 毕宁 陈会 吴继雄[1] CHEN Ying;FANG Liang;LIU Zheng-ai;BI Ning;CHEN Hui;WU Ji-xiong(Huanggang Central Hospital,Hubei 438000,China)

机构地区：[1]黄冈市中心医院消化内科,湖北黄冈138000

出　　处：《中华医院感染学杂志》2020年第23期3607-3611,共5页Chinese Journal of Nosocomiology

基　　金：湖北省科研基金资助项目(2018DM012510)。

摘　　要：目的分析乙肝肝硬化患者血清乙型肝炎病毒脱氧核糖核酸(Hepatitis B Virus-Deoxyribonucleic Acid,HBV-DNA)载量与热休克蛋白70(Heat shock protein 70,Hsp70)、癌胚抗原(Carcino-embryonic antigen,CEA)及甲胎蛋白(α-fetoprotein,AFP)水平的相关性。方法将2016年5月-2019年5月在黄冈市中心医院消化内科就诊且符合纳入与排除标准的120例乙肝肝硬化患者作为研究对象,分别应用荧光定量PCR法检测HBV-DNA载量,酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测血清HSP70、CEA、AFP水平;比较不同HBV-DNA载量的乙肝肝硬化患者血清HSP70、CEA及AFP水平;并应用协方差分析校正混杂因素影响,Pearson相关性分析血清HBV-DNA载量与校正后的HSP70、CEA及AFP水平的相关性。结果 120例乙肝肝硬化患者中,HBV-DNA载量<5log10拷贝/ml 64例,平均(3.65±0.29)log10拷贝/ml;≥5log10拷贝/ml 56例,平均(5.98±0.28)log10拷贝/ml;其中HBV-DNA载量≥5log10拷贝/ml的乙肝肝硬化患者HSP70低于HBV-DNA载量<5log10拷贝/ml患者,CEA、AFP均高于HBV-DNA载量<5log10拷贝/ml患者(P<0.05);校正后不同HBV-DNA载量的乙肝肝硬化患者HSP70、CEA、AFP水平经Bonferroni检验差异仍有统计学意义(P<0.05);Pearson相关性分析显示HBV-DNA载量与HSP70、CEA、AFP均有显著相关性(r=-0.525、0.637、0.409,均P<0.01)。结论乙肝肝硬化患者血清HBV-DNA载量与HSP70显著负相关,与CEA、AFP呈正相关关系。OBJECTIVE To analyze the correlation between serum hepatitis B virus-deoxyribonucleic acid(HBV-DNA) load and levels of heat shock protein 70(Hsp70), carcinoembryonic antigen(CEA) and alpha-fetoprotein(AFP) in 120 patients with hepatitis B cirrhosis. METHODS A total of 120 patients with hepatitis B cirrhosis who were admitted to the Huanggang Central Hospital between May 2016 and May 2019 were accordance with the inclusion criteria and recruited as the research subjects. The HBV-DNA load, levels of HSP70, CEA and AFP were measured by fluorescent quantitative PCR and enzyme-linked immunosorbent assay(ELISA), respectively. Levels of serum HSP70, CEA and AFP in patients with hepatitis B cirrhosis and different HBV-DNA loads were compared. The effect of confounding factors was corrected by covariance analysis, and the correlation between serum HBV-DNA load and adjusted levels of HSP70, CEA and AFP were analyzed by Pearson correlation analysis. RESULTS In the 120 patients with hepatitis B cirrhosis, HBV-DNA load was smaller than 5 log10 copies/ml in 64 cases, with an average of(3.65±0.29) log10 copies/ml. The load was bigger than 5 log10 copies/ml or equal in 56 cases, with an average of(5.98±0.28) log10 copies/ml. HSP70 levels in patients with HBV-DNA load <5 log10 copies/ml were significantly lower than that in patients with HBV-DNA load ≥5 log10 copies/ml, whereas CEA and AFP were higher than that in patients with HBV-DNA load ≥5 log10 copies/ml. The Bonferroni test showed that there were significant differences in levels of HSP70, CEA and AFP between patients with different HBV-DNA loads after correction(P<0.05). Pearson correlation analysis showed that there was an correlation between HBV-DNA load and HSP70, CEA and AFP(r=-0.525, 0.637, 0.409, all P<0.01). CONCLUSION The serum HBV-DNA load in patients with hepatitis B cirrhosis was negatively correlated with HSP70, and positively correlated with CEA and AFP.

关 键 词：乙肝肝硬化 乙型肝炎病毒脱氧核糖核酸 热休克蛋白70 癌胚抗原 甲胎蛋白 

分 类 号：R512.6+2[医药卫生—内科学]