Respiratory Control by Phox2b-expressing Neurons in a Locus Coeruleus–pre B?tzinger Complex Circuit  

作　　者：Na Liu Congrui Fu Hongxiao Yu Yakun Wang Luo Shi Yinchao Hao Fang Yuan Xiangjian Zhang Sheng Wang 

机构地区：[1]Department of Physiology,Hebei Medical University,Shijiazhuang 050017,China [2]Department of Physiology,Cangzhou Medical College,Cangzhou 061000,China [3]School of Nursing,Hebei Medical University,Shijiazhuang 050000,China [4]Hebei Key laboratory of Vascular Homeostasis and Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease,Shijiazhuang 050000,China

出　　处：《Neuroscience Bulletin》2021年第1期31-44,共14页神经科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31971058 and 31571174);the Youth Fund for Scientific and Technological Research in Higher Education Institutions of Hebei Province(QN2019019);the Youth Science and Technology Talent Support Program of Natural Science in Hebei Medical University(CYQD201907)。

摘　　要：The locus coeruleus(LC) has been implicated in the control of breathing.Congenital central hypoventilation syndrome results from mutation of the paired-like homeobox 2 b(Phox2 b) gene that is expressed in LC neurons.The present study was designed to address whether stimulation of Phox2 b-expressing LC(Phox2 b~(LC)) neurons affects breathing and to reveal the putative circuit mechanism.A Cre-dependent viral vector encoding a Gqcoupled human M3 muscarinic receptor(hM3 Dq) was delivered into the LC of Phox2 b-Cre mice.The hM3 Dqtransduced neurons were pharmacologically activated while respiratory function was measured by plethysmography.We demonstrated that selective stimulation of Phox2 b~(LC) neurons significantly increased basal ventilation in conscious mice.Genetic ablation of these neurons markedly impaired hypercapnic ventilatory responses.Moreover,stimulation of Phox2 b~(LC) neurons enhanced the activity of preBotzinger complex neurons.Finally,axons of Phox2 b~(LC) neurons projected to the preBotzinger complex.Collectively,Phox2 b~(LC) neurons contribute to the control of breathing most likely via an LC-preBotzinger complex circuit.

关 键 词：Locus coeruleus Phox2b Hypercapnic ventilatory response CHEMORECEPTOR Neural circuit 

分 类 号：Q42[生物学—神经生物学]