杜仲无糖组培生根微环境控制技术  

Study on Microenvironment Control Technology of Sugar-free Root Tissue Culture of Eucommia ulmoides

作　　者：王立 黄宏健 李小燕 谭沛涛 胡杨 叶棠 梁慧霞 陈小玲 WANG Li;HUANG Hongjian;LI Xiaoyan;TAN Peitao;HU Yang;YE Tang;LIANG Huixia;CHEN Xiaoling(Xinhui Forestry Science Institute,Jiangmen,Guangdong 529100;Guangdong NanFang Institute of Tenchnology,Jiangmen,Guangdong 529100)

机构地区：[1]江门市新会区林业科学研究所,广东江门529100 [2]广东南方职业学院,广东江门529100

出　　处：《热带农业工程》2020年第6期91-94,共4页Tropical Agricultural Engineering

基　　金：广东省江门市科学技术局基础与理论科学研究项目(No.2018030100120015802)。

摘　　要：为研究杜仲组培苗生根阶段在无糖组培微环境下CO_(2)调控方式,研究了不同CO2浓度及最高通气量,筛选出最佳CO_(2)浓度及最大换气量。结果显示,CO2浓度为1400、1600μmol/mol时,生根率和生长状态较好(出根时间分别是31、30 d;最长主根根长分别是6.26、6.20 cm;根数平均为3.53、6.20条;生根率均为100%),最大通气量为1400 mL/min时,杜仲无糖组培苗生长最好(株高达7.35 cm,平均叶片面积达2.94 cm2,最长主根根长为6.25 cm,根数达4.07条)。In order to study the regulation mode of CO_(2) in tissue culture microenvironment of Eucommia ulmoides during rooting stage.7 concentrations of CO_(2) and 7 levels of maximum ventilation were studied,and the best CO2 concentration and maximum ventilation were selected.The results showed that the rooting rate and growth state are better when CO_(2) concentration is 1400 or 1600μmol/mol(rooting time is 31 days and 30 days respectively,and the longest main root length is 6.26 cm and 6.20 cm,respectively.The root number is the best when CO_(2) concentration is 1400μmol/mol,with an average of 3.53;the root number isreach up to the average of 6.20 when CO_(2) concentration is 1600μmol/mol.The rooting rate is all 100%.When the maximum aeration rate is1400 mL/min,Eucommia ulmoides tissue culture seedlings grow best(the plant height is7.35 cm,the average leaf area is 2.94 cm_(2),the longest main root length is 6.25 cm,the root number is 4.07).

关 键 词：杜仲 无糖组培 CO_(2)浓度 通气量 

分 类 号：S567.19[农业科学—中草药栽培]