多指示剂标记适体探针卡那霉素传感器的电化学行为与分析性能  

Electrochemical behavior and analytical performance of kanamycin biosensor based on multi-indicator modified aptamer probe

作　　者：崔陪 韩贤达 向阳可佳 俞志刚[1,2] CUI Pei;HAN Xianda;XIANG Yangkejia;YU Zhigang(College of Chemical and Environmental Engineering,Harbin University of Science and Technology,Harbin 150040;Post-Doctoral Research Center of Chongqing Key Laboratory of Inorganic Special Functional Materials,College of Chemistry and Chemical Engineering,Yangtze Normal University,Filling 408100)

机构地区：[1]哈尔滨理工大学化学与环境工程学院,哈尔滨150040 [2]无机特种功能材料重庆市重点实验室博士后科研工作站,长江师范学院化学化工学院,涪陵408100

出　　处：《分析试验室》2021年第1期48-53,共6页Chinese Journal of Analysis Laboratory

基　　金：重庆市自然科学基金(cstc2018jcyjAX0637);长江师范学院科研启动基金计划(2017KYQD122)资助。

摘　　要：以三分子亚甲基蓝(MB)标记适体探针为分子识别元件,构建了一种卡那霉素生物传感器。该传感器采用目标物诱导探针构型变化的信号转导机制。以Au-S化学法将适体探针自组装于金电极表面形成稳定的单分子层传感界面,利用交流伏安法和循环伏安法考察了传感过程的基础电化学行为和传感器分析性能。在优化条件下,相比于单分子MB标记,该传感器的界面电活性和电流输出效率获得了明显提升。在PBS中用于目标物卡那霉素检测,检出限为3.3 nmol/L,电流响应平衡时间为15 s,同时具有高选择性、强抗污检测能力和好的平行构建稳定性。该方法检测过程免样品前处理,无需添加辅助试剂。A kanamycin biosensor was fabricated using a DNA probe modified by 3 molecule methylene blue(MB)as the molecular recognition element.The sensor signaling mechanism was based on the change in probe conformation induced by the target binding.The 3 MB-modified aptamer probe was self-assembled on the Au electrode surface to form a stable sensing interface by Au-S chemistry.The basic electrochemical behavior and analytical performance of sensor system were investigated using alternate current voltammetry and cyclic voltammetry.Under the optimized conditions,the interfacial electroactivity and the current output efficiency were significantly improved compared with those obtained from MB-modified DNA probe.When used for the detection of target kanamycin,the sensor exhibited a low detection limit of 3.3 nmol/L and super-fast sensing speed with the current response equilibrium time of only 15 s,and showed high selectivity,strong anti-fouling ability,and good parallel fabrication stability.The method avoids sample pretreatment and does not need to add additional reagent.

关 键 词：适体传感器 卡那霉素 DNA探针 电化学行为 分析性能 

分 类 号：O657.37[理学—分析化学]