牛血红蛋白催化荧光猝灭法测定痕量甲巯咪唑  

Determination of methimazole based on the quenching by bovine hemoglobin catalyzed fluorescence

作　　者：凌绍明 欧阳辉祥 覃登攀 谢宇奇 LING Shaoming;OUYANG Huixiang;QING Dengpan;XIE Yuqi(Guangxi Colleges and Universities Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi,College ofChemistry and Environment Engineering,Baise University,Baise 533000)

机构地区：[1]广西高校桂西区域生态环境分析和污染控制重点实验室,百色学院化学与环境工程学院,百色533000

出　　处：《分析试验室》2021年第1期91-94,共4页Chinese Journal of Analysis Laboratory

摘　　要：在NH3·H2O-NH4Cl缓冲溶液中,牛血红蛋白催化H2O2氧化L-酪氨酸产生荧光,甲巯咪唑对该荧光体系具有较强的猝灭作用。据此建立了酶催化荧光猝灭法测定痕量甲巯咪唑的方法。结果表明,甲巯咪唑含量在0.986~14.8μg/L范围内与荧光强度变化值呈线性关系,其线性回归方程为ΔF405 nm=50.923ρ+191.45,相关系数(R^(2))为0.9945,检出限为为0.32μg/L。方法用于测定药品中甲巯咪唑含量,测定结果的RSD为4.7%,回收率92%~96.3%。Based on bovine hemoglobin can catalyzethe oxidation of L-tyrosine by H2O2 to produce a fluorescence in NH3·H2O-NH4Cl buffer solwtion and the system’s fluorescence is quenched strongly in the presence of methimazole,a new method for trace methimazole detection was established.The results showed that the fluorescence intensities linearly decreased with the increasing of methimazole concentrations in the range of 0.986-14.8μg/L,with the linear regression equation ofΔF405 nm=50.923ρ+191.45,the correlation coefficient of 0.9945,and the detection limit of 0.32μg/L.The method was applied to determine the methimazole in medicine,with the RSD of 4.7%,and the recovery of 92%-96.3%.

关 键 词：甲巯咪唑 荧光猝灭法 L-酪氨酸 牛血红蛋白 

分 类 号：O657.3[理学—分析化学]