miRNAs小鼠哮喘模型中的作用初探  

Role of miRNAs in the model of asthma in mice

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作  者:张冬姣 徐浩[1] ZHANG Dongjiao;XU Hao(Pediatric Department,Central Hospital Affiliated to Shenyang Medical Colloge,Shenyang 110024,China)

机构地区:[1]沈阳医学院附属中心医院儿科,沈阳110024

出  处:《中国中西医结合儿科学》2021年第1期1-4,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

摘  要:目的探讨小鼠哮喘模型中miRNAs的差异表达情况。方法将24只BALB/c小鼠随机分为正常对照组、卵清蛋白处理组、地塞米松治疗组各8只,使用血细胞分析仪检测每组肺泡灌洗液中嗜酸性粒细胞(EOS)、嗜中性粒细胞(NEU)、巨噬细胞(MAC)和淋巴细胞(LYM)的表达情况,通过ELISA的方法检测每组肺泡灌洗液中白细胞介素-4(IL-4)、IL-6和IL-13的表达情况。miRNAs芯片分析不同组别肺组织中差异性表达miRNAs的情况,统计分析miR-455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的相关性。结果卵清蛋白处理组中EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达显著高于正常对照组,地塞米松治疗组与卵清蛋白处理组相比各种因子表达下调。卵清蛋白处理组多个miRNAs表达发生变化,miR-455在卵清蛋白处理组上调显著。miR-455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达呈正相关。结论miR-455在卵清蛋白诱导的小鼠哮喘模型中显著上调。Objective To investigate the differential expression of miRNAs in asthma model of mice.Methods BALB/c mice were randomly divided into normal control group,asthma group(ovalbumin treatment group)and treatment group(dexamethasone treatment group),8 mice in each group.Eosinophil(EOS),neutrophil(NEU),macrophage(MAC)and lymphocyte(LYM)in BALF of each group were detected by hematology analyzer,and the expression of IL-4,IL-6 and IL-13 in BALF of each group were detected by ELISA.miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues;the correlation between miR-455 and EOS,NEU,MAC,LYM,IL-4,IL-6 and IL-13 was statistically analyzed.Results The expression of EOS,NEU,MAC,LYM,IL-4,IL-6 and IL-13 in the asthma group was significantly higher than that in the control group,and the expression of various factors was down-regulated in treatment group compared with asthma group.The expression of multiple miRNAs in asthma group changed,and miR-455 was up-regulated significantly in asthma group.miR-455 was positively correlated with the expression of EOS,NEU,MAC,LYM,IL-4,IL-6 and IL-13.Conclusion miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.

关 键 词:哮喘 微小RNA 炎症因子 细胞因子 

分 类 号:R725.6[医药卫生—儿科]

 

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