HPLC测定鱼饲料中硝基呋喃类违禁药物的方法研究  

Determination of 4 Nitrofurans in Fish Feed by HPLC

作　　者：杨俊华[1] 陈海燕[1] 马静[1] 谢荣国[1] 马兴海 Yang Junhua;Chen Haiyan;Ma Jing;Xie Rongguo;Ma Xinghai(Ningxia Veterinary Drugs and Fodder Inspection Institute,Yinchuan,Ningxia 750011;Animal's Sanitation Administration,Haiyuan,Ningxia 755200)

机构地区：[1]宁夏回族自治区兽药饲料监察所,宁夏银川750011 [2]海原县动物卫生监督所,宁夏海原755200

出　　处：《宁夏农林科技》2020年第11期55-57,共3页Journal of Ningxia Agriculture and Forestry Science and Technology

摘　　要：建立了饲料中呋喃唑酮、呋喃它酮、呋喃西林和呋喃妥因4种硝基呋喃类药物的高效液相色谱分析方法。此试验条件下硝基呋喃类药物的线性范围是0.5~10μg/mL,线性相关系数为0.9993~0.9997,回收率范围为72%~93%,变异系数为0.7%~3.4%,检测限为0.3 mg/kg,定量限为1.0 mg/kg。本试验操作简单,标准曲线线性好,准确率和重复性高,符合方法要求。A high performance liquid chromatography(HPLC)method was developed for the determination of furazolidone,furantoin,furazolin and nitrofurantoin in feed.The linear range of nitrofurans was 0.5~10μg/mL,the linear correlation coefficient was 0.9993~0.9997,the recovery was 72%~93%,the coefficient of variation was 0.7%~3.4%,the detection limit was 0.3 mg/kg,and the limit of quantification was 1.0 mg/kg.The test is simple and the standard curve has good linearity,high accuracy and repeatability,which meets the requirements of the method.

关 键 词：饲料 硝基呋喃类药物 高效液相色谱法 残留检测方法 

分 类 号：S816.17[农业科学—饲料科学]