HPLC切换波长法测定益肾灵颗粒(无蔗糖)中特女贞苷、淫羊藿苷、补骨脂素和异补骨脂素的含量  

Content Determination of Specnuezhenide,Psoralen,Ang-elicin and Icariin in Yishenling Granules(Sucrose-free)by HPLC Wavelength Switching Method

作　　者：方慧祥 崔庆德 吕晋 FANG Huixiang;CUI Qingde;Lü Jin(Qujing center for Food and Drug control,Qujing 655000,China)

机构地区：[1]云南省曲靖市食品药品检验检测中心,云南曲靖655000

出　　处：《中国民族民间医药》2021年第3期28-32,共5页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立高效液相色谱法同时测定益肾灵颗粒(无蔗糖)中特女贞苷、淫羊藿苷、补骨脂素和异补骨脂素含量的方法。方法:色谱柱为依利特C_(18)柱(250 mm×4.6 mm,5μm),柱温为30℃;流动相为甲醇-乙腈-水,梯度洗脱;流速为1.0 mL·min^(-1);检测波长为224 nm、270 nm、246 nm。结果:特女贞苷、、淫羊藿苷补骨脂素、异补骨脂素的进样量分别在0.03401~0.8501μg(r=0.9997,n=7)、0.1006~2.5151μg(r=1.0000,n=7)、0.01916~0.4790μg(r=0.9999,n=7)、0.02020~0.5051μg(r=0.9999,n=7)范围内与其峰面积积分值呈良好的线性关系;精密度、重复性、稳定性试验的RSD均<1.0%;平均加样回收率分别为98.5%、100.6%、99.4%、98.9%,RSD分别为0.50%、0.93%、0.49%、0.65%(n=6)。结论:本方法专属性强、精密度高、分离度及重现性好,可用于测定益肾灵颗粒(无蔗糖)中特女贞苷、淫羊藿苷、补骨脂素和异补骨脂素的含量。Objective To establish a method for content determination of Specnuezhenide,Psoralen,Angelicin and Icariin in Yishenling granules simultaneously.Methods The determination was performed on Yilite C_(18) column(250 mm×4.6 mm,5μm)with mobile phase consisted of methanol-acetonitrile-water at the flow rate of 1.0 mL/min.The column temperature was 30℃,and the detection wavelengths were set at 224 nm,246 nm and 270 nm.Results The method achieved good linearity in the ranges of 0.03401~0.8501μg(r=0.9997,n=7)for Specnuezhenide,0.01916~0.4790μg(r=0.9999,n=7)for Psoralen,0.02020~0.5051μg(r=0.9999,n=7)for Angelicin and 0.1006~2.5151μg(r=1.0000,n=7)for Icariin.The RSDs of precision,reproducibility and stability tests were lower than 1.0%.The average recoveries were 98.5%,99.4%,98.9%and 100.6%,the RSDs were 0.50%,0.49%,0.65%and 0.93%(n=6).Conclusions The method is specific,precise and reproducible.It can be used for the content determination of Specnuezhenide,Psoralen,Angelicin and Icariin in Yishenling granules.

关 键 词：高效液相色谱法 益肾灵颗粒(无蔗糖) 特女贞苷 淫羊藿苷 补骨脂素 异补骨脂素 含量测定 

分 类 号：R284.2[医药卫生—中药学]