低氧对斑马鱼胚胎发育和红细胞生成的抑制作用  

Inhibition effects of hypoxia on embryonic development and erythropoiesis in zebrafish

作　　者：楚璐萌 田子颖 崔蕊 吴娇[2] 于海川 CHU Lumeng;TIAN Ziying;CUI Rui;WU Jiao;YU Haichuan(School of Laboratory Medicine,Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine,Xinxiang Medical University,Xinxiang 453003,China;School of Pharmacy,Xinxiang Medical University,Xinxiang 453003;the Second Affiliated Hospital of Xinxiang Medical University,Henan Key Laboratory of Biological Psychiatry,Xinxiang Medical University,Xinxiang 453002;Zhengzhou No.7 People’s Hospital of Henan Province,Zhengzhou 450000)

机构地区：[1]新乡医学院医学检验学院,河南省分子诊断与医学检验技术协同创新中心,河南新乡453003 [2]新乡医学院药学院,河南新乡453003 [3]新乡医学院第二附属医院,河南省生物精神病学重点实验室,河南新乡453002 [4]河南省郑州市第七人民医院,郑州450000

出　　处：《中国实验动物学报》2021年第1期1-8,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(31301135);河南省高等学校重点科研项目资助计划(21A320018);河南省高校科技创新人才支持计划(17HASTIT047);河南省高等学校青年骨干教师资助计划(2016GGJS-103);新乡医学院精神神经医学学科群支持计划(2016PNKFKT-08),新乡医学院产学研合作项目(2017CXY-2-14);河南省生物精神病学重点实验室开放课题(ZDSYS2016001);研究生创新支持计划资助项目(YJSCX201811Z)。

摘　　要：目的本文以斑马鱼(Danio rerio)为研究对象,探讨了低氧对早期胚胎发育、造血分化和红系分化的影响。方法选取受精后12 h的斑马鱼胚胎,随机分为两组,以常氧组为对照组,低氧组为实验组,实时观察斑马鱼胚胎发育形态学的变化;通过联苯胺染色、邻联茴香胺染色、AO染色及瑞氏吉姆萨染色观察红细胞的生成及形态学变化;并通过Real time PCR检测了斑马鱼胚胎造血相关基因的表达情况。结果与常氧相比,低氧降低了斑马鱼胚胎卵黄囊的营养消耗,抑制了色素细胞的形成,减慢了心率,延缓了斑马鱼胚胎的孵化,观察和分析了低氧对红细胞产生和成熟的抑制作用。结论低氧延缓了斑马鱼胚胎发育,抑制了红细胞的产生和成熟。Objective The vertebrate model of zebrafish(Danio rerio)was employed to explore the effects of hypoxia on early embryonic development,hematopoietic differentiation,and erythroid differentiation.Methods At 12 h post-fertilization,zebrafish embryos were randomly divided into two groups.The normoxic group was used as the control group,and the hypoxic group was used as the experimental group.The morphological changes of zebrafish embryos were observed in real-time.Erythropoiesis and morphological changes were observed by benzidine,O-dianisidine,acridine orange,and May-Grunwald Giemsa staining.Real time PCR was used to analyze hematopoietic gene expression in zebrafish embryos.Results Hypoxia reduced nutritional consumption of the yolk sac,inhibited the formation of pigment cells,slowed down the heart rate,and delayed the hatching of zebrafish embryos.Inhibitive effects of hypoxia on the production and maturity of red blood cells were observed.Conclusions Hypoxia delays zebrafish embryonic development and inhibits the production and maturity of red blood cells.

关 键 词：斑马鱼 低氧 胚胎发育 造血分化 红细胞生成 

分 类 号：Q95-33[生物学—动物学]