角叉菜胶诱导的慢性血栓小鼠模型的建立  

Preliminary establishment of chronic thrombus model in mice

作　　者：韩子逸 沈诗怡 张金鑫 周燕[1] 赵昕[1] 刘明江[1,2] 徐霄龙 HAN Ziyi;SHEN Shiyi;ZHANG Jinxin;ZHOU Yan;ZHAO Xin;LIU Mingjiang;XU Xiaolong(School of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009;Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏省动物重要疫病与人畜共患病防控协同创新中心,江苏扬州225009 [3]首都医科大学附属北京中医医院,北京100010

出　　处：《中国实验动物学报》2021年第1期78-84,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：江苏省扬州大学研究生科技创新(XKYCX_137);江苏省高校优势学科建设工程资助项目。

摘　　要：目的采用不同浓度的角叉菜胶溶液连续腹腔注射的方式处理小鼠,筛选慢性血栓小鼠模型的条件。方法将70只ICR雄性小鼠随机分为7组,每组10只,即:空白组对照组、0.02%剂量组、0.04%剂量组、0.06%剂量组、0.08%剂量组、0.10%剂量组、0.20%剂量组。空白组小鼠腹腔注射生理盐水(0.01 mL/g),其余组腹腔注射相应浓度的角叉菜胶溶液(0.01 mL/g),每天注射1次;各组小鼠饲养于(20±1)℃室温下并自由采食、饮水。每天观察各组出现黑尾的情况(黑尾率、黑尾长度);出栓即采样;采血检测血凝四项,检测结肠组织与血浆中氧化应激指标及炎性因子指标。结果0.06%剂量组小鼠第5天出栓2只,第6天出栓8只;与空白对照组相比,0.06%剂量组小鼠FIB含量显著升高(P<0.05),APTT显著缩短(P<0.05),TT显著延长(P<0.05),PT极显著延长(P<0.01);0.06%剂量组结肠组织与血浆中SOD、GSH-Px均极显著降低(P<0.01)、MDA显著升高(P<0.05);0.06%剂量组结肠组织与血浆中TNF-α、IL-1β显著升高(P<0.05)、IL-10显著降低(P<0.05)。结论初步得出室温为(20±1)℃条件下,0.06%的角叉菜胶溶液(0.01 mL/g)连续腹腔注射6 d(每天1次)是建立慢性血栓小鼠模型的最适条件。Objective To screen the conditions of a chronic thrombus mouse model after injecting a carrageenan solution at various concentrations intraperitoneally.Methods Seventy ICR male mice were randomly divided into seven groups with 10 mice in each group:blank(control),0.02%,0.04%,0.06%,0.08%,0.10%,and 0.20%groups.Mice in the blank group were intraperitoneally injected with normal saline(0.01 m L/g),whereas mice in the other groups were intraperitoneally injected with carrageenan solutions at the corresponding concentration(0.01 m L/g)once a day.Mice in each group kept at(20±1)°C and had free access to food and water.The black-tailed conditions(black-tailed rate and length)of each group were observed each day.An outlet plug was used for sampling.Blood samples were collected to detect blood clots,and indicators of oxidative stress and inflammatory factors in colon tissues and plasma were detected.Results In the 0.06%dose group,two mice were thrombotic on 5 d and eight mice were thrombotic on day 6.Compared with the blank group,FIB content in the 0.06%dose group was increased significantly(P<0.05),APTT was shortened significantly(P<0.05),TT was prolonged significantly(P<0.05),and PT was prolonged significantly(P<0.01).SOD and GSH-Px in colon tissue and plasma of the 0.06%dose group were decreased significantly(P<0.01),and MDA was increased significantly(P<0.05).The colon tissue and plasma levels of TNF-αand IL-1βin the 0.06%dose group were increased significantly(P<0.05),and those of IL-10 were decreased significantly(P<0.05).Conclusions A0.06%carrageenan solution(0.01 m L/g)injected intraperitoneally for 6 d(once a day)at(20±1)°C is the optimal condition to establish a mouse model of chronic thrombosis.

关 键 词：慢性血栓 小鼠 角叉菜胶 氧化应激 炎性因子 

分 类 号：Q95-33[生物学—动物学]