无菌动物无菌检测的探讨  

Discussion on aseptic tests of germ-free animals

作　　者：李永军 李兰娟[1] LI Yongjun;LI Lanjuan(State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Hangzhou 310003,China)

机构地区：[1]浙江大学医学院附属第一医院传染病诊治国家重点实验室,国家感染性疾病临床医学研究中心,感染性疾病诊治协同创新中心,杭州310003

出　　处：《中国实验动物学报》2021年第1期110-115,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家重点基础研究发展计划(2013CB531400)。

摘　　要：近年来,肠道微生物在人类的健康和疾病中的应用受到了广泛关注。无菌动物在肠道微生态中的模型应用越来越广泛,为人类的生理、病理研究提供了非常有效的研究工具。为保证实验动物的质量,必须对无菌动物频繁的进行无菌检测。但无菌检测受到诸多因素的干扰。目前传统的检测方法主要是细菌、真菌培养及革兰染色镜检。随着高通量测序的发展,现在新增了一种分子生物学检测技术(PCR),利用16S rRNA进行细菌的鉴定。但这些方法都存在一定的局限性。本文将对这些方法的利弊及影响因素进行简要探讨。In recent years,the role of intestinal microorganisms in human health and diseases has been widely researched.The application of a germ-free animal model in intestinal microecology is extensive and this model provides a very effective research tool for human physiological and pathological research.To ensure the quality of experimental animals,aseptic tests must be frequently performed.However,sterility tests are affected by many factors.At present,the traditional detection method are mainly bacterial and fungal culture and Gram staining.With the development of highthroughput sequencing,polymerase chain reaction method have been used to identify bacteria using 16 S rRNA.However,these method have some limitations.In this paper,we discuss the advantages and disadvantages of these method and the influencing factors.

关 键 词：无菌动物 无菌检测 细菌培养 16S rRNA PCR 

分 类 号：Q95-33[生物学—动物学]