基于UPLC-Q-TOF-MS技术的阿司匹林抵抗血清代谢组学研究  

Study on Serum Metabolomics of Aspirin Resistance Based on UPLC-Q-TOF-MS Technology

作　　者：蒋志涛[1] 吕玲燕 韩怡[1] 倪磊 JIANG Zhitao;LV Lingyan;HAN Yi;NI Lei(Department of Pharmacy,Zhangjiagang Hospital Affiliated to Nanjing University of Chinese Medicine,Zhangjiagang 215600,China)

机构地区：[1]南京中医药大学附属张家港医院药学部,张家港215600

出　　处：《药学与临床研究》2021年第1期27-30,共4页Pharmaceutical and Clinical Research

基　　金：江苏省药学会-天晴医院药学基金课题(Q2018169)。

摘　　要：目的:发现阿司匹林抵抗(AR)的潜在生物标志物,探讨AR可能机理。方法:以金黄地鼠的AR模型为研究对象,采用基于超高效液相色谱-飞行时间质谱(UPLC-Q-TOF-MS)技术的代谢组学方法考察血清中内源性代谢物的变化,采用主成分分析、偏最小二乘法及置换检验分析等,确定AR血清中潜在生物标志物。结果:与对照组相比,AR组金黄地鼠血清中有脱氧胆酸、硬脂酸、松香酸、谷氨酰胺、肌酸等11个特异性代谢分子。结论:脱氧胆酸、硬脂酸等11个成分可作为AR的潜在生物标志物,提示AR致病机理可能与脂肪酸生物合成、二次胆汁酸生物合成、氨基酸代谢有关,这为进一步研究药物改善AR奠定基础。Objective:To explore potential serum biomarkers characterizing aspirin resistance(AR)and explore the possible mechanism of AR.Methods:Based on the established AR model in hamsters,a UPLC-Q-TOF-MS-based metabolomics method was carried out to analyze the changes of endogenous metabolites in serum.The principal component analysis,partial least square method and permutation test analysis were used to determine the potential serum biomarkers in the AR group.Results:Compared with the control group,eleven specific metabolites,including deoxycholic acid,stearic acid,abietic acid,glutamine and creatine,were found in the serum of the AR group.Conclusion:There are eleven components including deoxycholic acid and stearic acid as potential biomarkers of AR,suggesting that the pathogenesis of AR may be related to fatty acid biosynthesis,secondary bile acid biosynthesis and amino acid metabolism,laying a foundation for further research on drugs to improve AR.

关 键 词：代谢组学 阿司匹林抵抗 超高效液相色谱-飞行时间质谱 潜在生物标志物 

分 类 号：R969.1[医药卫生—药理学]