NIR-Ⅱ Excitation and NIR-I Emission Based Two-photon Fluorescence Lifetime Microscopic Imaging Using Aggregation-induced Emission Dots  

作　　者：LIU Wen ZHANG Yuhuang QI Jj QIAN Jun TANG Ben Zhong 

机构地区：[1]State Key Laboratory of Modern Optical Instrumentations,Centre for Optical and Electromagnetic Research,College of Optical Science and Engineering,International Research Center for Advanced Photonics,Zhejiang University,Hangzhou,310058,P.R.China [2]Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates,South China University of Technology,Guangzhou,510640,P.R.China [3]Department of Chemistry,The Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction,Institute for Advanced Study and Department of Chemical and Biological Engineering and Institute of Molecular Functional Materials,The Hong Kong University of Science and Technology,Clear Water Bay,Kowloon,Hong Kong,P.R.China

出　　处：《Chemical Research in Chinese Universities》2021年第1期171-176,共6页高等学校化学研究（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.61735016,61975172);the Natural Science Foundation of Zhejiang Province of China(No.LR17F050001);the Fundamental Research Funds for the Central Universities of China(No.2020-KYY-511108-0007);the Open Fund of Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregate,South China University of Technology,China(No.2019B030301003).

摘　　要：Near-infrared(NIR)lights are powerful tools to conduct deep-tissue imaging since NIR-Ⅰ wavelengths hold less photon absorption and NIR-Ⅱ wavelengths serve low photon scattering in the biological tissues compared with visible lights.Two-photon fluorescence lifetime microscopy(2PFLM)can utilize NIR-Ⅱ excitation and NIR-Ⅰ emission at the same time with the assistance of a well-designed fluorescent agent.Aggregation induced emission(AIE)dyes are famous for unique optical properties and could serve a large two-photon absorption(2PA)cross-section as aggregated dots.Herein,we report two-photon fluorescence lifetime microscopic imaging with NIR-Ⅱ excitation and NIR-Ⅰ emission using a novel deep-red AIE dye.The AIE-gens held a 2PA cross-section as large as 1.61×10^(4)GM at 1040 nm.Prepared AIE dots had a two-photon fluorescence peak at 790 nm and a stable lifetime of 2.2 ns under the excitation of 1040 nm femtosecond laser.The brain vessels of a living mouse were vividly reconstructed with the two-photon fluorescence lifetime information obtained by our home-made 2PFLM system.Abundant vessels as small as 3.17µm were still observed with a nice signal-background ratio at the depth of 750µm.Our work will inspire more insight into the improvement of the working wavelength of fluorescent agents and traditional 2PFLM.

关 键 词：NEAR-INFRARED Brain imaging Aggregation-induced emission Two-photon fluorescence lifetime microscopic imaging 

分 类 号：O413[理学—理论物理]