临床疑似病例中火鸡组织滴虫的检测及其在脏器分布规律  

Detection and distribution of Histomonas meleagridis in tissues and organs of clinically suspected cases of turkeys

作　　者：戎杰 孔令明 陈乔光 侯照峰[1,2] 刘丹丹 陶建平[1,2] 许金俊 RONG Jie;KONG Lingming;CHEN Qiaoguang;HOU Zhaofeng;LIU Dandan;TAO Jianping;XU Jinjun(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《畜牧与兽医》2021年第1期113-116,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金面上项目(31772727);扬州市重点研发计划(现代农业)(YZ2017051);江苏高校优势学科建设二期工程资助;扬州大学“青蓝工程”资助(2018年度);扬州大学专业学位研究生实践创新计划(XSJCX18_075)。

摘　　要：为了探明临床病鸡中火鸡组织滴虫的脏器分布规律,本研究收集21例临床疑似病鸡的321个组织器官,采用PCR技术对样品中的虫体DNA进行了检测。结果:组织脏器中肝脏和盲肠的阳性检出率最高,分别为57.1%和52.4%;其次为回肠、脾脏、肾脏、肌胃、胰腺和脑,阳性率分别为43.8%、40.0%、40.0%、37.5%、35.7%和33.3%;心、肺、法氏囊和其他肠道组织等检出率较低;胆囊未检测到。结论:火鸡组织滴虫主要感染肝脏和盲肠,其次为回肠、脾脏、肾脏、肌胃、胰腺和脑,其他脏器虫体分布较少。In order to identify the distribution law of Histomonas meleagridis in clinically suspected turkeys,a total of 321 tissue samples from 21 cases were detected by PCR.The results showed that the positive rates of the organs of liver and cecum of the birds were the highest,at 57.1%and 52.3%,respectively,followed by those of the ileum,spleen,kidney,gizzard,pancreas and brain at 43.8%,40.0%,40.0%,37.5%,35.7%and 33.3%,respectively.The detection rates of heart,lung,bursa of Fabricius and other intestinal tissues were low,and no positive rate of the gallbladder was detected.In conclusion,Histomonas meleagridis is most infectious to the turkey organs of cecum and liver,followed by those of ileum,spleen,kidney,gizzard,pancreas and brain,and was seldom infectious to to the other organs.

关 键 词：火鸡组织滴虫 PCR 分布规律 

分 类 号：S852.72[农业科学—基础兽医学]