基于代谢组学研究二苯乙烯苷在对乙酰氨基酚诱导小鼠肝损伤中的保护作用  

Tetrahydroxy stilbene glycoside protects mice from acetaminophen-induced liver injury:study based on metabonomics

作　　者：高岩[1,2,3] 李均童[2,4] 吴青林 李林 张兰 GAO Yan;LI Juntong;WU Qinglin;LI Lin;ZHANG Lan(Department of Pharmacology,Xuanwu Hospital,Capital Medical University,Phase I Clinical Research Center,Beijing 100053,China;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China;Key Laboratory of Molecular Pharmacology and Drug Evaluation,Ministry of Education,Yantai University,Yantai 264005,Shandong,China;Science and Technology Innovation Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China)

机构地区：[1]首都医科大学宣武医院药学部药物研究室,Ⅰ期临床研究中心,北京100053 [2]中国医学科学院北京协和医学院药物研究所,北京100050 [3]烟台大学分子药理和药物评价教育部重点实验室,烟台264005 [4]广州中医药大学科技创新中心,广州510405

出　　处：《中国临床药理学与治疗学》2021年第2期161-166,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：北京市医院管理中心“登峰”计划专项经费资助(DFL20190803);首都科技领军人才培养工程(Z191100006119017);北京市博士后工作经费资助项目(2020-ZZ-001)。

摘　　要：目的:通过非靶向代谢组学等研究方法探讨二苯乙烯苷(tetrahydroxy stilbene glucoside,TSG)对乙酰氨基酚(acetaminophen,APAP)造成急性肝损伤的保护作用。方法:采用随机分组方式将SPF级C57BL/6小鼠分为APAP模型组和TSG干预组,每组15只。灌胃给予小鼠TSG连续7 d后,一次性腹腔注射APAP进行造模,6 h后取肝脏组织。结果:H&E染色、MDA和SOD检测表明单次APAP注射会造成肝脏损伤,TSG干预会降低肝脏受损程度。代谢产物检测结果显示,与APAP模型组相比,TSG组的ABC转运体、胆碱代谢、中心碳代谢、半乳糖、丙氨酸类氨基酸代谢等变化显著。结论:TSG通过改善脂质过氧化和能量代谢紊乱等过程抵御APAP导致的急性肝损伤。AIM:To explore the pharmaceutical effects of tetrahydroxy stilbene glycoside(TSG)on acute liver injury induced by acetaminophen(APAP)using method of non-targeted metabonomics.METHODS:SPF C57BL/6 mice were randomly divided into the group of APAP-induced model and the group of TSG intervention groups(n=15).After intragastric administration of TSG for 7 days,the mice were injected once by APAP via intraperitoneal injection and the livers were taken 6 hours later.RESULTS:H&E staining,MDA and SOD tests showed that the injection of APAP could cause hepatic injury,but TSG could reduce the severity of liver injury.The results of metabolite detection showed that there were significant changes in ABC transporter,choline metabolism,central carbon metabolism,galactose and alanine amino acid metabolism in TSG groups compared with APAP model group.CONCLUSION:TSG protects against acute liver injury induced by APAP,mainly by improving lipid peroxidation and disorder of energy metabolism.

关 键 词：二苯乙烯苷 对乙酰氨基酚 代谢组学 急性肝损伤 

分 类 号：R965.2[医药卫生—药理学]