改良输尿管置入膀胱尿路重建法建立小鼠肾移植模型  

Modified technique of ureter implantation for urinary tract reconstruction in murine renal transplantation model

作　　者：余意 毕梓荣 王东[2] 李军[1] 江启锋 杨诗聪[4] 傅茜[1] 刘龙山[1] 王长希[1] Yu Yi;Bi Zirong;Wang Dong;Li Jun;Jiang Qifeng;Yang Shicong;Fu Qian;Liu Longshan;Wang Changxi(Organ Transplant Center,First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China;Department of Clinical Laboratory Medicine,First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China;Department of Pathology,Guangzhou Huayin Medical Laboratory Center,Guangzhou 510663,China;Department of Pathology,First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院器官移植科,广州510080 [2]中山大学附属第一医院检验科,广州510080 [3]广州华银医学检验中心肾脏病理室,510663 [4]中山大学附属第一医院病理科,广州510080

出　　处：《中华器官移植杂志》2021年第1期48-51,共4页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金(81870511、81670680、81700655、81300623);广东省科技计划项目(2015B020226002、2017A020215012);广东省自然科学基金(2018A030313016);广东省基础与应用基础研究基金(2020A1515010884);广州市科技计划项目(201903010058、201803040011);广东省器官捐献与移植免疫重点实验室(2013A061401007、2017B030314018);器官移植国际合作基地建设(2015B050501002)。

摘　　要：目的探讨小鼠肾移植术中输尿管膀胱置入的改良方法。方法30对BALB/c小鼠同品系间行肾移植术,供肾均为左肾,应用双套管法建立供肾血液循环,由传统的先置入后固定包埋的方法,改良为先固定,再置入,最后包埋的方法,记录手术时间、存活率及并发症发生情况。结果供肾血管分离、灌注、切除时间(25±6)min,热缺血时间(10±6)s,冷缺血时间(25±5)min。受体手术:动静脉分离时间(12±5)min,静脉套置时间(7±1)min,动脉套置时间(7±1)min,输尿管膀胱吻合时间(13±2)min,切除右肾时间(5±1)min,关腹(5±1)min。总手术时间(77±3)min。成功建立26例移植模型,手术成功率86.7%(26/30)。结论改良后的输尿管置入膀胱尿路吻合术更简单快捷,小鼠肾移植术后尿路并发症发生率低,更有利于初学者掌握。Objective To explore the application value of modified technique of ureter implantation in murine renal transplantation.Methods Thirty left donor kidneys from BALB/c mice was transplanted into syngeneic mice.Cuff technique was applied for anastomosing kidney artery and vein.The procedure of ureter-bladder anastomoses shifted from implication-fixation-embedding to fixation-implication-embedding.Operative duration,recipient survival rate and complications were recorded.Results Time for separating vessels,perfusion and excision of donor graft was(25±3)min,(10±6)s for warm ischemia and(25±5)min for cold ischemia.Time for separating recipient vessels was(12±5)min,(7±1)min for arterial anastomosis,(7±1)min for venous anastomosis,(13±2)min for ureter-bladder anastomosis,(5±1)min for right kidney excision and(5±1)min for abdominal closure.Operative duration was(77±3)min.Twenty-six recipients survived over 3 months.The successful operative rate was 86.7%.Conclusions With a shorter learning curve,modified technique of ureter implantation is easier and faster so as to reduce the postoperative incidence of urinary tract complications during murine renal transplantation.

关 键 词：肾移植 输尿管膀胱吻合术 小鼠 

分 类 号：R699.2[医药卫生—泌尿科学] R-332[医药卫生—外科学]