CRISPR/Cas9系统在水稻分子育种中的应用  

Application of CRISPR/Cas9 system in rice molecular breeding

作　　者：刘欣欣 李赫 卜庆云[2] 田晓杰[2] 王臻昱[2] 何明良 唐佳琦 李秀峰[2] 孟威 LIU Xinxin;LI He;BU Qingyun;TIAN Xiaojie;WANG Zhenyu;HE Mingliang;TANG Jiaqi;LI Xiufeng;MENG Wei(College of Life Science,Northeast Forestry University,Harbin 150060,China;Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,China;College of Life Science,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150060 [2]中国科学院东北地理与农业生态研究所,黑龙江哈尔滨150081 [3]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《土壤与作物》2021年第1期18-26,共9页Soils and Crops

基　　金：黑龙江省自然科学基金(面上项目)(C2018065);国家自然科学基金青年基因(31801327)。

摘　　要：CRISPR/Cas9是目前广泛应用的基因编辑技术,其操作方便、效率高、成本低。水稻作为全球粮食安全的战略作物,优质高产的水稻培育尤为重要,CRISPR/Cas9技术为水稻分子育种带来了突破。本文回顾了基因编辑技术的发展,详细介绍了CRISPR/Cas9系统的结构、组成及原理,重点阐述了该技术在水稻分子育种中的应用,探讨CRISPR/Cas9系统存在的问题以及其在分子育种的发展前景。CRISPR/Cas9 gene-editing technology is a widely used gene-editing system,which is easy-to-use,efficient and cost-effective.Rice is a strategic crop for global food security.Thus cultivation of high quality and high-yielding rice is of great importance.The CRISPR/Cas9 technology has brought a breakthrough in rice molecular breeding.This paper reviews the development of gene-editing technology,introduces the structure,composition and principle of the CRISPR/Cas9 gene-editing system,focuses on the applications of the technology in rice molecular breeding and discusses the problems and perspectives of its applications in rice molecular breeding.

关 键 词：基因编辑 CRISPR/Cas9 水稻 分子育种 

分 类 号：Q784[生物学—分子生物学] S511[农业科学—作物学]