消银解毒饮通过cdc7抑制角质细胞增殖作用的动物实验研究  

Animal experimental study on the mechanism of Xiaoyinjiedu Decoction inhibiting keratinocyte proliferation through cdc7

作　　者：韩露 周扬 段行武[1] 陈兵[1] 方如男 李建红[1] HAN Lu;ZHOU Yang;DUAN Xingwu;CHEN Bing;FANG Ru'nan;LI Jianhong(Department of Dermatology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《中国麻风皮肤病杂志》2021年第3期148-152,共5页China Journal of Leprosy and Skin Diseases

摘　　要：目的:明确消银解毒饮通过细胞分裂周期蛋白7(cdc7)对小鼠银屑病模型角质细胞增殖的干预作用。方法:将20只健康雄性BALB/c小鼠随机分为对照组、银屑病模型组、消银解毒饮组与cdc7抑制剂TAK-931组,每组5只,除对照组外,每组背部涂抹咪喹莫特诱导银屑病模型,连续5天。对照组和银屑病模型组予蒸馏水10 mL/kg,消银解毒饮组和cdc7抑制剂TAK-931组小鼠分别予消银解毒饮23.4 g/kg,TAK-9312 mg/kg灌胃处理,每天2次,连续5天。每日观察皮损情况进行PASI评分;HE染色观察皮损组织形态学改变及表皮厚度;免疫组化法检测皮损表皮中cdc7表达。结果:银屑病模型建模成功。与银屑病模型组相比,消银解毒饮组与TAK-931组小鼠背部皮损PASI评分低,表皮增生程度较轻,炎性细胞浸润较少(均P<0.05);消银解毒饮组表皮cdc7阳性表达细胞较银屑病模型组明显减少(P<0.05)。结论:消银解毒饮可能通过下调cdc7表达,抑制角质形成细胞的过度增殖,改善咪喹莫特诱导的小鼠银屑病样皮损。Objective:To determine the effect of Xiaoyinjiedu Decoction on the proliferation of keratinocytes through the cell division cycle protein 7(cdc7)in the mouse model of psoriasis.Methods:Twenty healthy male BALB/c mice were randomly divided into the control group,psoriasis model group,Xiaoyinjiedu Decoction group and cdc7 inhibitor TAK-931 group with 5 mice in each group.In addition to the control group,imiquimod was applied to the back of the mice for 5 days in other groups to induce psoriasis model.The mice in the control group and psoriasis model group were treated with distilled water 10 mL/kg,twice a day,for 5 days.The mice in the Xiaoyinjiedu Decoction group and cdc7 inhibitor TAK-931 group were treated with Xiaoyinjiedu Decoction 23.4 g/kg and cdc7 inhibitor TAK-9312 mg/kg twice a day,for 5 days.The PASI scores were assessed.The morphological changes of lesions and the thickness of the epidermis were measured by HE staining.The level of cdc7 in epidermis of skin lesion was assessed by immunohistochemistry.Results:The mouse psoriasis model was constructed successfully.Compared to the psoriasis model group,the PASI scores of lesions in the Xiaoyinjiedu Decoction group and TAK-931 group were lower,the hyperplasias were milder,the inflammatory cells were less(P s<0.05).The level of positive cells of cdc7 in epidermis in the Xiaoyinjiedu Decoction group was significantly lower than that in the psoriasis model group(P<0.05).Conclusion:Xiaoyinjiedu Decoction can inhibit the excessive proliferation of keratinocytes,which may through down-regulating the expression of cdc7.

关 键 词：消银解毒饮 细胞分裂周期蛋白7 银屑病 

分 类 号：R285.5[医药卫生—中药学]