甘蔗抗褐锈病新基因抗感病池构建及SSR多态性引物筛选  

New Brown Rust Resistance Gene of Sugarcane:Construction of Resistant and Susceptible Gene Pools and SSR Polymorphic Primers Screening

作　　者：王晓燕[1] 单红丽[1] 张荣跃[1] 仓晓燕[1] 王长秘 李文凤[1] 尹炯[1] 罗志明[1] 黄应昆 Wang Xiaoyan;Shan Hongli;Zhang Rongyue;Cang Xiaoyan;Wang Changmi;Li Wenfeng;Yin Jiong;Luo Zhiming;Huang Yingkun(Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement,Kaiyuan Yunnan 661699)

机构地区：[1]云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室,云南开远661699

出　　处：《中国农学通报》2021年第6期97-103,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目“滇蔗茅对甘蔗褐锈病的抗性遗传规律分析及抗性基因定位”(31660419);国家现代农业产业技术体系(糖料)建设专项资金“甘蔗真菌性病害防控岗位科学家”(CARS-170303);云岭产业技术领军人才培养项目“甘蔗有害生物防控”(2018LJRC56);云南省技术创新人才培养对象项目(202005AD160012);云南省现代农业产业技术体系建设专项资金“云南省甘蔗产业技术体系植物保护与病害研究岗位科学家”(YNGZTX-4-92)。

摘　　要：由黑顶柄锈菌(Puccinia melanocephala H.Sydow&P.Sydow)引起的甘蔗褐锈病是危害中国甘蔗生产的主要病害之一。为了鉴定和发掘抗褐锈病新基因,防止褐锈病爆发流行和保证甘蔗安全生产,本研究以杂交组合‘粤糖03-393’בROC 24’抗感分离真实性F1代群体为材料,构建抗感基因池,合成449对引物对抗感亲本及抗感基因池进行抗、感连锁SSR分子标记筛选。结果表明,25对引物在抗感亲本间有多态性,其中4对引物(SMC236CG、SCESSR0928、SCESSR0636、SCESSR2551)在抗感亲本及抗感池间有多态性,初步判定这4个SSR标记在染色体上的位点可能与抗褐锈病新基因存在连锁关系。研究结果为后续开展抗褐锈病新基因定位、为中国甘蔗褐锈病防控及抗病育种奠定了良好的基础。Brown rust caused by Puccinia melanocephala H.Sydow&P.Sydow is one of the main diseases that endanger sugarcane production in China.In order to identify and explore the new brown rust resistance gene,prevent the outbreaks of the disease and ensure the safe production of sugarcane,the authentic F1 hybrid population of cross combination‘Yuetang 03-393’בROC 24’was used to construct resistant/susceptible gene pools,and then 449 pairs of primers were synthesized to screen SSR(simple-sequence repeat)markers that linked to resistance and susceptibility in the parental lines and the resistant/susceptible genetic pools in this study.The results showed that 25 pairs of primers were polymorphic between the parents,and among them four pairs of primers(SMC236CG,SCESSR0928,SCESSR0636,and SCESSR2551)were polymorphic between the parental lines and the resistant/susceptible genetic pools.It preliminarily indicated that the loci of these four SSR markers on chromosome might have linkage relationship with a new brown rust resistance gene.The results of this study establish a good foundation for the mapping of new brown rust resistance genes in the future,and for controlling sugarcane brown rust and breeding disease resistance varieties in China.

关 键 词：甘蔗 褐锈病 抗病基因 抗感病池 SSR标记 

分 类 号：S432.44[农业科学—植物病理学]