四种药用植物内生真菌的分离及抗单胺氧化酶的活性研究  

Isolation and anti-monoamine oxidase activity of endophytic fungi from four medicinal plants

作　　者：苟文博 杨中铎[1] 周格格 GOU Wen-bo;YANG Zhong-duo;ZHOU Ge-ge(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China)

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050

出　　处：《湖北农业科学》2021年第3期56-59,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金资助项目(21762027)。

摘　　要：从栓翅卫矛(Euonymus phellomanus)、甘西鼠尾草(Salvia przewalskii)、刺五加(Acanthopanax senticosus)和黄海棠(Hypericum ascyron)4种药用植物的根及茎中分离并鉴定内生真菌,对其发酵产物乙酸乙酯提取物进行体外抗单胺氧化酶活性筛选,并采用柱层析法、波谱法分离鉴定活性菌株中的成分。结果显示,从这些植物中共分离得到20株内生真菌。其中,菌株SJ-2、WJ-3、HJ-1抗单胺氧化酶活性抑制率分别为83.8%、84.2%、71.9%。根据菌落形态特征及分子生物学鉴定,菌株SJ-2被鉴定为镰孢属(Fusarium)真菌,内生真菌WJ-3被鉴定为青霉菌属(Penicillium)真菌,HJ-1被鉴定为链格孢属(Alternaria)真菌。从菌株SJ-2中分离得到3个化合物,分别鉴定为Enniatin B、Monomethylsulochrin、Fumitremorgin C。Endophytic fungi were isolated and identified from the roots and stems of four medicinal plants,Euphorbia pulcherrima,Sal⁃via przewalskii,Acanthopanax senticosus and Hypericum ascyron.The anti-monoamine enzyme activity of ethyl acetate extract of fer⁃mentation product from these endophytes was screened,and chemical constituents from these endophytes were isolated by column chromatography and spectroscopy methods.The results showed that 20 endophytic fungi strains were obtained.Among them,the inhi⁃bition rate of anti-monoamine oxidase activity of SJ-2,WJ-3,HJ-1 was 83.8%,84.2%,71.9%,respectively.According to the colo⁃ny characteristics and morphological characteristics,the endophytic fungus SJ-2 was identified as Fusarium sp.,WJ-3 was identified as Penicillium sp.,and HJ-1 was identified as Alternaria sp..Three compounds,Enniatin B,Monomethylsulochrin,Fumitremorgin C were isolated from strain SJ-2.

关 键 词：药用植物 内生真菌 次级代谢产物 生物活性 单胺氧化酶抑制剂 

分 类 号：R284.1[医药卫生—中药学] Q93-330[医药卫生—中医学]