外源5-氨基乙酰丙酸对干旱胁迫下黄瓜种子萌发及幼苗生长的影响  

Effects of exogenous 5-aminolevulinic acid on seed germination and seedling growth of cucumber under drought stress

作　　者：邓硕真 刘惠军[1] 王洪芹[1] 王红艳[1] 张倩茹 张梅[1] DENG Shuo-zhen;LIU Hui-jun;WANG Hong-qin;WANG Hong-yan;ZHANG Qian-ru;ZHANG Mei(Yantai Institute,China Agricultural University,Yantai 264670,Shandong,China)

机构地区：[1]中国农业大学烟台研究院,山东烟台264670

出　　处：《湖北农业科学》2021年第3期70-74,78,共6页Hubei Agricultural Sciences

基　　金：烟台市科技攻关项目(2017ZH097);中国农业大学烟台研究院重点资助项目(YT201901);大学生科研训练项目(U20183021)。

摘　　要：以津耘五号黄瓜(Cucumis sativus L.)为试验材料,以20%PEG-6000模拟干旱胁迫,研究不同浓度外源5-氨基乙酰丙酸(5-ALA)对干旱环境下黄瓜种子发芽及幼苗生长的影响。结果表明,20%PEG-6000模拟的干旱严重抑制了黄瓜种子的萌发,不同浓度的5-ALA可以缓解干旱胁迫对黄瓜种子萌发的影响,随着5-ALA浓度的升高,种子的发芽率、发芽指数、活力指数出现了先上升后下降的趋势,其中0.5 mg/L为最佳浓度。20%PEG-6000模拟的干旱抑制了黄瓜幼苗的生长发育,适宜浓度的5-ALA可以显著提高黄瓜幼苗的抗旱性,其中0.5 mg/L 5-ALA处理下,黄瓜幼苗的干重、壮苗指数、根系活力、可溶性糖含量、游离脯氨酸含量、总叶绿素含量、类胡萝卜素含量分别比干旱环境下提高99.13%、104.51%、70.42%、40.79%、163.46%、38.59%、126.92%。Taking Jinyun NO.5 cucumber(Cucumis sativus L.)as the test materials,the experiment was conducted to study the effects of different concentrations 5-aminolevulinic acid(5-ALA)on seed germination and seedling growth of cucumber under the condition of 20%PEG-6000 drought stress.The results showed that,drought environment seriously inhibited the germination of cucumber seeds,and different concentrations of 5-ALA can alleviate the effects of drought stress on cucumber seed germination.With the in⁃crease of 5-ALA concentration,the germination rate,germination index and vigor index of seeds first increased and then decreased.Among them,0.5 mg/L is the best concentration.Drought environment inhibits the growth of cucumber seedlings.Appropriate concen⁃tration of 5-ALA can significantly increase the drought resistance of cucumber seedlings.At a concentration of 0.5 mg/L,the dry weight,strong seedling index,root activity,soluble sugar content,free proline content,total chlorophyll content and carotenoid con⁃tent of cucumber seedlings respectively increased by 99.13%,104.51%,70.42%,40.79%,163.46%,38.59%,126.92%compared with the dry environment.

关 键 词：黄瓜(Cucumis sativus L.) 外源5-氨基乙酰丙酸 种子萌发 干旱胁迫 

分 类 号：S642.2[农业科学—蔬菜学]