地耳草总黄酮提取工艺优化与含量测定  

Optimization of extraction process and determination of total flavonoids in Hypericum japonicum Thumb

作　　者：吴冰 莫婷婷 陆梅元 曾超[2] 赵凤仙 WU Bing;MO Ting-ting;LU Mei-yuan;ZENG Chao;ZHAO Feng-xian(Liuzhou hospital of traditional Chinese medicine,Liuzhou 545001,Guangxi,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China;Guangxi District Food and Drug Review and Inspection Center,Nanning 530029,China)

机构地区：[1]柳州市中医医院,广西柳州545001 [2]广西中医药大学第一附属医院,南宁530023 [3]广西壮族自治区食品药品审评查验中心,南宁530029

出　　处：《湖北农业科学》2021年第3期116-119,共4页Hubei Agricultural Sciences

基　　金：全国中药特色技术传承人才培训项目(2015481601003);广西高校中青年教师科研基础能力提升项目(2019KY0341);广西中医药大学青年基金项目(2019QN036)。

摘　　要：采用回流提取法提取地耳草(Hypericum japonicum Thumb.)总黄酮,通过单因素试验,考察提取方法、提取溶剂体积分数、提取体积、提取时间对总黄酮含量的影响,采用正交试验,优化提取工艺条件,筛选出地耳草总黄酮的最佳提取工艺。结果表明,地耳草总黄酮的最佳提取工艺为75%乙醇、料液比1∶30(g/mL)、回流提取时间1.0 h。该方法重复性好、稳定,能有效测定地耳草总黄酮的含量,为该药材的质量标准研究提供一定的科学依据。The total flavonoids of Hypericum japonicum Thumb.was extracted by reflux extraction method.The effects of extraction method,extraction solvent concentration,extraction volume,and extraction time on the total flavonoid content of medicinal materials were investigated by single factor test.Orthogonal experiments were used to optimize the extraction process conditions and screen the best extraction technology of total flavonoids from Hypericum japonicum Thumb..The results showed that the optimal extraction process of total flavonoids from Hypericum japonicum Thumb.was 75%ethanol,the material-liquid ratio was 1∶30(g/mL),and the reflux ex⁃traction time was 1.0 h.This method in this experiment has good repeatability,and the method is stable and feasible,and can effective⁃ly determine the content of total flavonoids in Hypericum japonicum Thumb.,and provide a certain scientific basis for the study of the quality standard of this medicinal material.

关 键 词：地耳草(Hypericum japonicum Thumb.) 总黄酮 提取工艺 紫外可见-分光光度法 

分 类 号：R284.2[医药卫生—中药学]