低温诱导海蜇螅状体横裂生殖的分子信号调控机制初探  

Preliminary study on the molecular signaling regulation mechanism in Rhopilema esculentum polyps strobilation induced by low temperature

作　　者：吴晓雅 龚先宇 谭树亮 甄毓[1,2,4] 米铁柱 WU Xiao-ya;GONG Xian-yu;TAN Shu-liang;ZHEN Yu;MI Tie-zhu(Laboratory for Marine Ecology and Environmental Science,Qingdao 266100,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;College of Marine Life Science,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Ecology and Environmental Science,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266071,China)

机构地区：[1]海洋环境与生态教育部重点实验室,山东青岛266100 [2]中国海洋大学环境科学与工程学院,山东青岛266100 [3]中国海洋大学海洋生命学院,山东青岛266003 [4]青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室,山东青岛266071

出　　处：《海洋环境科学》2021年第1期66-72,共7页Marine Environmental Science

基　　金：国家重点研发计划课题项目(2017YFC1404402,2017YFC1404404);国家自然科学基金项目(41606135);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2016ASKJ02)。

摘　　要：海蜇生活史是由有性生殖的水母体世代和无性生殖的螅状体世代组成的。海蜇螅状体的无性繁殖是决定水母种群大小的主要繁殖方式。而作为螅状体无性生殖方式之一的横裂生殖是螅状体世代向水母体世代转变的唯一途径,其发生主要受温度调控。本研究对海蜇螅状体进行短期低温培养,比较分析低温诱导组和对照组螅状体转录组的差异,初步探究低温诱导对海蜇螅状体横裂生殖的影响及横裂生殖过程中的分子调控机制。研究结果显示,海蜇螅状体横裂生殖过程中出现1655条差异基因,分属于细胞生长、增殖、分化等信号转导通路,如转化生长因子信号通路(transforming growth factor beta,TGF-β)、丝裂原活化蛋白激酶信号通路(mitogen-activated protein kinase,MAPK)、细胞外分泌蛋白信号通路(wingless-int,Wnt)以及细胞周期介导的信号通路(Cell Cycle)等。在低温诱导下,上述信号通路均有所响应,但总体来看Wnt信号通路被抑制,其他3条通路被激活。通过分析,本研究初步展示了参与低温诱导海蜇螅状体横裂生殖的分子调控途径,为深入揭示海蜇横裂生殖的分子调控机制提供思路。The life cycle of Rhopilema esculentum involves pelagic medusa and benthic polyp.Asexual reproduction is the main reproduction method of R.esculentum.As one of the asexual reproductions,the strobilation is the only way to transform polyps to medusae,which is mainly regulated by temperature.In order to investigate the effects of low temperature induction and the molecular regulation mechanism in the strobilation,R.esculentum polyps were cultured at low temperature for a short period,and the transcriptome differences between the low temperature group and control group were compared and analyzed.Results showed that there were 1655 differential expressed genes in the strobilation,which involved in TGF-β(transforming growth factor beta),MAPK(mitogen-activated protein kinase),Wnt(wingless-int)and Cell Cycle signaling pathways.Under the induction of low temperature,the above signaling pathways responded,but the Wnt signaling pathway was inhibited and the other three pathways were activated.This study preliminarily demonstrates the molecular regulation pathways involved in low temperature-induced R.esculentum polyps,which provides a help for further investigation of molecular regulation mechanism in strobilation of R.esculentum.

关 键 词：海蜇 横裂生殖 低温 转录组 信号通路 

分 类 号：Q178.53[生物学—水生生物学]