自制冷冻片微型载体(Strawtop)微量精液冻存效果的实验研究  

Experimental study on low volume sperm cryopreservation using Strawtop micro-carrier

作　　者：王荣祥 喻乔 彭秋平 曹少锋 张彩霞 刘宇 周怡雯 薛松果 Wang Rongxiang;Yu Qiao;Peng Qiuping;Cao Shaofeng;Zhang Caixia;Liu Yu;Zhou Yiwen;Xue Songguo(Center for Reproductive Medicine,Shanghai East Hospital,Tongji University School of Medicine,Shanghai 200120,China;Reproductive Medicine Center,Hunan Provincial Maternal and Child Health Care Hospital,Changsha 410008,China)

机构地区：[1]同济大学附属东方医院生殖医学中心,上海200123 [2]湖南省妇幼保健院,长沙410008

出　　处：《中华生殖与避孕杂志》2020年第12期1006-1012,共7页Chinese Journal of Reproduction and Contraception

基　　金：上海市市级医院新兴前沿技术联合攻关项目(SHDC12015122);上海市自然科学基金(09ZR1417000)。

摘　　要：目的对自制冷冻片微型载体(Strawtop)法与麦管法冷冻保存人微量精液的冻存效果进行系统评价。方法采用前瞻性研究分析2018年5月1日至2018年6月30日期间于同济大学附属东方医院生殖医学中心行卵胞质内单精子显微注射(ICSI)助孕治疗的患者捐献的部分精液样本,比较自制Strawtop法与麦管法冷冻人精子复苏后的冷冻复苏率、DNA碎片指数(DNA fragmentation index,DFI)、超显微结构和受精能力与胚胎发育情况。结果Strawtop法冻存人精子的冷冻复苏率(46.8%±17.1%)显著高于麦管法(23.1%±13.7%,P=0.001);与冷冻前(18.9%±11.6%)相比,麦管法(33.5%±15.0%)冷冻精子复苏后的DFI显著增加(P=0.019),而Strawtop法冻融入精子的DFI(23.4%±11.7%)与冷冻前相比差异无统计学意义(P=0.375);Strawtop法对冷冻精子超显微结构的损伤低于麦管法;Strawtop法冻融精子、麦管法冻融精子和新鲜精子的正常受精率、卵裂率和受精后48 h(第2日)的优质胚胎率差异均无统计学意义(P>0.05)。结论Strawtop法冷冻人微量精液冷冻复苏率高于麦管法,复苏精子的损伤较小,且复苏后无需离心洗涤即可直接用于ICSI,具有较高的临床应用价值。Objective To evaluate the cryopreservation effect of the home-made Strawtop micro-carrier method and Straw method for cryopreservation of human low volume sperm.Methods A prospective study was performed to analyze the post-thaw results of cryopreserved human sperm using the home-made Strawtop method and Straw method.Compare the survival rate,sperm DNA fragmentation index(DFI),ultrastructure,fertilization ability and embryo developmental competence of donated semen samples from the patients who underwent intracytoplasmic sperm injection(ICSI)treatment in Center for Reproductive Medicine,Shanghai East Hospital,Tongji University School of Medicine during the period from May 1,2018 to June 30,2018.Results The survival rate of Strawtop group(46.8%±17.1%)was significantly higher than that of Straw group(23.1%±13.7%,P=0.001).Compared with fresh sperm(18.9%±11.6%),Straw method(33.5%±15.0%)significantly increased the sperm DFI(P=0.019),while Strawtop method didn't(23.4%±11.7%,P=0.375).The sperm ultrastructure of Strawtop group was superior to that of Straw group.There was no difference among Strawtop,Straw and fresh group in normal fertilization rates,cleavage rates and day 2 high-quality embryo rates.Conclusion For human low volume sperm cryopreservation,the Strawtop method was simple and convenient.The survival rate of Strawtop method was higher than that of straw method and the sperm could be used for ICSI directly after thawing.The strawtop method is worth to be widely used in clinical practice.

关 键 词：无精子症 严重少精子症 微量精液冷冻 麦管法 Strawtop法 

分 类 号：R698.2[医药卫生—泌尿科学]