大黄素调节细胞粘附与侵袭抑制鼠黑色素瘤转移  

Emodin suppresses metastasis of murine melanoma via regulation of adhesion and invasion of melanoma cells

作　　者：何振辉[1,2] 蔡坤泰 翁闪凡[1] 林鹏 魏琼 HE Zhen-hui;CAI Kun-tai;WENG Shan-fan;LIN Peng;WEI Qiong(.Department of Laboratory Medicine,Foshan University,Foshan 528000,China;Foshan Stomatology Hospital,Foshan University,Foshan 528000,China;Department of Stomatology,Foshan University,Foshan 528000,China;Department of Clinical Laboratory,Foshan Nanhai District Fifth People's Hospital,Foshan 528231,China)

机构地区：[1]佛山科学技术学院医学检验系,广东佛山528000 [2]佛山科学技术学院附属口腔医院佛山市口腔医院,广东佛山528000 [3]佛山科学技术学院口腔医学系,广东佛山528000 [4]佛山市南海区第五人民医院检验科,广东佛山528231

出　　处：《佛山科学技术学院学报（自然科学版）》2021年第1期26-31,共6页Journal of Foshan University(Natural Science Edition)

基　　金：国家级大学生创新创业训练计划资助项目(201811847014);佛山市医学类科技攻关资助项目(2017AB002071,201108064);广东省中医药局建设中医药强省课题资助项目(20111057);佛山科学技术学院医药工程学院挑战杯“金种子”资助项目。

摘　　要：目的研究大黄素(Emodin,Emo)对鼠源性黑色素瘤细胞B16F10转移相关能力的影响。方法利用Methyl Thiazolyl Tetrazolium法检测Emo对B16F10细胞存活的影响,细胞粘附实验检测Emo对B16F10细胞粘附玻连蛋白(VN)、纤连蛋白(FN)的影响,基质胶(matrigel)侵袭实验检测Emo对B16F10细胞侵袭重组基底膜的影响,划痕实验检测Emo对B16F10细胞迁移的影响。利用尾静脉注射法观察Emo对血循环中B16F10细胞粘附鼠肺组织和实验性转移的影响。结果Emo显著抑制B16F10细胞体外侵袭能力和粘附VN、FN的能力。Emo处理B16F10细胞24 h后,显著抑制B16F10细胞运动。Emo处理的B16F10细胞在C57BL/6小鼠肺中形成荧光斑点和肺转移结节的数量减少。结论Emo通过抑制B16F10细胞对细胞外基质(ECM)的粘附与侵袭抑制B16F10细胞肺转移。Objective To study the effect of emodin(Emo)on metastasis related ability of murine melanoma cell line B16F10.Methods Methyl thiazolyl tetrazolium assay was used to detect the effect of Emo on the survival of B16F10 cells.Cell adhesion assay was used to detect the effect of Emo on the adhesion of vitronectin(VN)and fibronectin(FN)of B16F10 cells.Matrigel invasion assay was used to detect the effect of Emo on the invasion of B16F10 cells into recombinant basement membrane.Scratch test was used to detect the effect of Emo on the migration of B16F10 cells.The effect of Emo on the adhesion of B16F10 cells to lung tissue and experimental metastasis was observed by tail vein injection.Results Emo significantly inhibited the invasion and adhesion of B16F10 cells to VN and FN in vitro.Emo significantly inhibited the movement of B16F10 cells after 24 h treatment.The number of fluorescent spots and metastatic nodules in the lung tissues of C57BL/6 mice treated with Emo decreased.Conclusion Emo can inhibit the lung metastasis of B16F10 cells by inhibiting the adhesion and invasion of B16F10 cells to ECM.

关 键 词：大黄素 粘附 侵袭 转移 黑色素瘤 

分 类 号：R282.71[医药卫生—中药学]