兔生前骨骼肌钝挫伤死后温度分布场变化的实验性研究  

Experimental study on changes of postmortem temperature distribution field of skeletal muscle blunt contusion before death in rabbits

作　　者：郑吉龙[1] 商庆发 张启翔 李海鹏 张文骥[1] 王云昕 Zheng Jilong;Shang Qingfa;Zhang Qixiang;Li Haipeng;Zhang Wenji;Wang Yunxin(Teaching and Research Section of Forensic Medicine,Criminal Investigation Police University of China;Technical Squadron of Criminal Investigation Brigade,Public Security Subbureau of Jiuyuan District,Public Security Bureau of Baotou)

机构地区：[1]中国刑事警察学院法医学系法医病理教研室,沈阳110035 [2]包头市公安局九原区公安分局刑事侦查大队技术中队,包头014060

出　　处：《重庆医科大学学报》2021年第2期201-205,共5页Journal of Chongqing Medical University

基　　金：公安部技术研究计划资助项目(编号:2016JSYJC56);大学生创新能力提升资助项目(编号:201910175075)。

摘　　要：目的:探讨利用红外热成像技术于死后早期检测个体骨骼肌钝挫伤热成像变化,以客观量化分析其随死亡时间变化的规律。方法:制备兔骨骼肌钝挫伤损伤模型后6 h,空气栓塞处死家兔,于损伤即刻、死后即刻、死后6 h、死后24 h、死后3 d、死后6 d应用红外热成像仪对家兔伤肢及健肢进行红外热成像及组织病理学检查,提取平均温度(average temperature,T_(mean))、最高温度(maximum temperature,T_(max))值,分析图像变化原因。结果:损伤即刻钝挫伤中心区红外热成像黄色区域面积增大、轮廓可见;死后即刻损伤区域红外热成像图像呈暗红色;死后6 h损伤轮廓呈圆形可辨别;死后24 h-6 d损伤区域轮廓不可识别;组内不同时间点红外热数据值有统计学差异(FT_(mean)=1162.040,PT_(mean)=0.000;FT_(Tmax)=1217.042,PT_(max)=0.000);家兔伤肢组T_(mean)、T_(max)较健肢组均降低且具有统计学差异(FT_(mean)=51.440,PT_(mean)=0.000;FT_(max))=69.808,PT_(max)=0.000);检测时间与组别存在交互作用(FT_(mean)=19.040,PT_(mean)=0.000;F_(Tmax)=15.769,PT_(max)=0.000);伤肢损伤区域T_(mean)、T_(max)值于死后6 h左右升高达峰值,之后逐渐下降,死后24 h、3 d、6 d逐渐趋于稳定。结论:个体死后不同经过时间检测生前骨骼肌钝挫伤,其损伤区域红外热成像及温度值参数变化具有一定时间规律性,为死后推断个体骨骼肌钝挫伤经过时间提供了无损的客观、量化检验方法。Objective:To investigate the thermal imaging change of skeletal muscle blunt contusion in the early postmortem stage by using infrared thermal imaging technology,so as to obtain the regularity of its change with the time of death via objective and quantitative analysis.Methods:Rabbit models with blunt contusion were established and killed by air embolization after 6 h.the skeletal muscle blunt injury model was prepared.Infrared thermal imaging and histopathology examination of injured and healthy limbs were performed by infrared thermal imager immediately after injury,immediately after death,6 h after death,24 h after death,3 d after death and 6 d after death.Average temperature(T_(mean))and maximum temperature(T_(max))were extracted and the reason of image change was analyzed.Results:Immediately after the injury,the yellow region of central blunt contusion was increased,with visible outline.Immediately after death,the infrared thermal image of the damaged area turned into dark red.Damage contour at 6 h after death was circular and distinguishable.The contour of damaged area was not recognizable during 24 h-6 d.There were significant differences in infrared thermal data values at different time points among groups(FT_(mean)=1162.040,PT_(mean)=0.000;FT_(max)=1217.042,PT_(max)=0.000).T_(mean) and Fmax in the injured limb group were lower than those in the healthy limb group,and there were significant differences(FT_(mean)=51.440,PT_(mean)=0.000;FT_(max)=69.808,PT_(max)=0.000).There was an interaction of detection time among groups(FT_(mean)=19.040,PT_(mean)=0.000;FT_(max)=15.769,PT_(max)=0.000).T_(mean) and T_(max) value in the injured limb were reached the peak at about 6 h after death,then gradually decreased,and gradually stabilized at 24 h,3 d and 6 d after death.Conclusion:Changes of infrared thermal imaging and temperature value parameter of skeletal muscle blunt contusion in different time after death have certain time regularity,which provides a nondestructive objective and quantitative testing method for p

关 键 词：法医病理学 骨骼肌钝挫伤 红外热成像 损伤经过时间 

分 类 号：R89[医药卫生—法医学]