2,4-二硝基氯苯对斑马鱼胚胎发育的毒性作用研究  

Developmental toxicity of 2,4-dinitrochlorobenzene in zebrafish embryos

作　　者：李雪青[1] 邢鸣鸾[1] 王晓峰[1] LI Xueqing;XING Mingluan;WANG Xiaofeng(Department of Environment and Health,Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou,Zhejiang 310051,China)

机构地区：[1]浙江省疾病预防控制中心环境与健康所,浙江杭州310051

出　　处：《预防医学》2021年第2期121-124,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：浙江省医药卫生科技计划项目(2017KY294)。

摘　　要：目的观察2,4-二硝基氯苯对斑马鱼胚胎发育的毒性作用。方法选取AB系野生型斑马鱼交配产卵,置于含Holt Buffer缓冲溶液的培养皿中培养。设置0.4、0.8、1.0、1.2、1.6、2.0μg/mL 6个剂量组,溶剂对照组和助溶剂对照组,每组20枚胚胎,暴露48、72和96 hpf(受精后小时),观察胚胎发育情况,计算胚胎死亡率、半数致死浓度(LC50)和畸形率。结果暴露48、72和96 hpf时,2,4-二硝基氯苯对斑马鱼胚胎的LC50分别为1.668、1.043和0.895μg/mL,随着时间延长逐渐下降。72 hpf后,当浓度达到2.0μg/mL时斑马鱼全部死亡。在0.4~2.0μg/mL范围内,48、72和96 hpf的斑马鱼死亡率随2,4-二硝基氯苯浓度增加而上升(均P<0.05),48 hpf的斑马鱼胚胎畸形率随着2,4-二硝基氯苯浓度增加而上升(P<0.05)。斑马鱼胚胎暴露于2,4-二硝基氯苯主要导致卵黄囊水肿、心包囊肿和脊柱弯曲等多种发育畸形。结论2,4-二硝基氯苯对斑马鱼胚胎具有致死和致畸作用,并影响斑马鱼的发育。Objective To investigate the developmental toxicity of 2,4-dinitrochlorobenzene in zebrafish embryos.Methods AB wild-type male and female zebrafish were selected to mate and spawn,then the eggs were cultured with Holt buffer solution.Six dose groups(0.4,0.8,1.0,1.2,1.6,2.0μg/mL),a solvent control group and a cosolvent control group,were set up with 20 embryos each.Malformations and death of embryos were observed at 48,72 and 96 hpf(hours post fertilization),the mortality and 50%lethal concentration(LC50)were also calculated.Results At 48,72 and 96 hpf,the LC50 of 2,4-dinitrochlorobenzene on zebrafish embryos were 1.668,1.043 and 0.895μg/mL,respectively,with a downward trend.After 72 hpf,when the concentration reached 2.0μg/mL,all the zebrafish died.In the range of 0.4-2.0μg/mL,the mortality of zebrafish at 48,72 and 96 hpf increased with the increase of 2,4-dinitrochlorobenzene concentration(all P<0.05);the malformation rate of zebrafish embryos at 48 hpf increased with the increase of 2,4-dinitrochlorobenzene concentration(P<0.05).Zebrafish embryos exposed to 2,4-dinitrochlorobenzene led to yolk sac edema,pericardial edema and spinal curvature.Conclusion 2,4-dinitrochlorobenzene can affect the development of zebrafish embryos,which will lead to lethal and teratogenic effects.

关 键 词：2 4-二硝基氯苯 斑马鱼胚胎 毒性作用 

分 类 号：R994.6[医药卫生—毒理学]