Optimization of Ultrasonic-enzymatic Extraction of Total Flavonoids from Cardamine tangutorum O.E.Schulz by Response Surface Methodology and Content Determination  

作　　者：Xiaoxia CAI Bochao LI Xuexue LI Hongna SU Wenbing LI Yuan LIU 

机构地区：[1]College of Pharmacy, Southwest Minzu University, Chengdu 610041, China [2]Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory, Chengdu 610225, China [3]Institute of Qinghai-Tibetan Plateau Research, Southwest Minzu University, Chengdu 610041, China [4]Ethnic Medicine Institute, Southwest Minzu University, Chengdu 610041, China

出　　处：《Medicinal Plant》2020年第6期36-41,共6页药用植物：英文版

基　　金：National Key Research and Development Program of China(2018YFC1708005);Applied Basic Research Plan of Sichuan Province(2018ZY0842);Fundamental Research Funds for the Central Universities(2020NGD01).

摘　　要：[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz.

关 键 词：Cardamine tangutorum O.E.Schulz Total flavonoid Response surface methodology Ultrasonic-enzymatic hydrolysis Extraction process 

分 类 号：R28[医药卫生—中药学]